Abstract: Fluorescent quantitative PCR technology is used for the detection of pathogenic microorganisms,gene expression,genome variation and polymorphism,etc.This technology and method has the advantages of high sensitivity,high specificity,fast,low sample requirements,and has been widely used in clinical diagnosis and livestock disease diagnosis.It is suggested that the construction of PCR laboratory should be based on the actual situation of dairy farm site,and the suitable PCR test area should be planned. Set up experimental equipment according to PCR testing requirements and dairy farm cost budget,allocate testing personnel and cultivate reserve personnel according to the expected testing sample quantity of dairy farm.On the basis of the establishment of strict operation specifications,strictly implement the experimental zoning management and disinfection process in the detection process,to avoid the production of aerosol pollution in the experiment process.

Key words: Yuan Sheng Tai Dairy Farm, fluorescence quantitative PCR, laboratory construction, practice and thinking