China Dairy ›› 2025, Vol. 0 ›› Issue (11): 15-23.doi: 10.12377/1671-4393.25.11.03

• CHINA EXCELLENT MILK PROJECT • Previous Articles     Next Articles

Effects of Heat Treatment Intensity on the Short-chain Fatty Acid Profile in Milk

LU Songcui1, XIAO Ran2,3, DU Shuang2,3, ZHAO Yankun4, ZHANG Yangdong1, ZHENG Nan1, WANG Jiaqi1, ZHANG Hongyan4, OU Guobing5, NIU Tianjiao2,3,*   

  1. 1 Institute of Animal Sciences,Chinese Academy of Agricultural Sciences; Key Laboratory of Dairy Quality and Digital Monitoring Technology,State Administration for Market Regulation,Beijing 100193;
    2 Mengniu Fresh Dairy Products (Tianjin) Co.,Ltd.,Tianjin 301700;
    3 Inner Mongolia Mengniu Dairy (Group) Co.,Ltd.,Hohhot Inner Mongolia 011500;
    4 Institute of Quality Standards & Testing Technology for Agro-Products,Xinjiang Academy of Agricultural Sciences,Urumqi Xinjiang 830091;
    5 Xinjiang Xiyuchun Dairy Co.,Ltd.,Changji Xinjiang 831201
  • Published:2025-12-22

Abstract: [Objective] This study aimed to investigate the effects of heat treatment intensity on the content and composition of short-chain fatty acids (SCFAs) in milk.[Method] Using raw milk from the same batch subjected to indirect heat treatments (75 °C/15 s,122 °C/4 s,137 °C/4 s) and direct heat treatment (130 °C/0.5 s),the contents of SCFAs and free short-chain fatty acids Free short-chain fatty acids (FSCFAs) were determined by gas chromatography-mass spectrometry (GC-MS),and differential analysis was performed.[Result] Both direct and indirect heat treatments led to unavoidable losses in total short-chain fatty acids (TSCFAs) and total free short-chain fatty acids (TFSCFAs) in raw milk. At the FSCFAs level,heat treatment alters the proportions of each FSCFAs,especially C2:0,C3:0 and C5:0,which may serve as potential markers for differentiating raw milk from heat-treated milk.[Conclusion] This study reveals the variation patterns of SCFAs and FSCFAs in milk under different heat treatment conditions,enriches the research data on the impact of heat treatment intensity on short-chain fatty acids in milk,and provides a reference for dairy quality evaluation and safety control.

Key words: heat treatment, short-chain fatty acids(SCFAs), milk, free short-chain fatty acids(FSCFAs)

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