Abstract: [Objective] The CHARM EPIC-PLUS commercial sterility detection system is a rapid microbial detection system based on the principle of adenosine triphosphate（ATP）bioluminescence. This system evaluates the microbial activity in samples by detecting the ATP content,achieving efficient determination of commercial sterility status. This study aims to verify the sensitivity and accuracy of the system in detection of milk beverages and improve the delivery time of the products.[Method] Bacillus subtilis,Escherichia coli and Saccharomyces cerevisiae were added to milk beverage. After incubation at（36±1）℃ for 48 hours,The CHARM EPIC-PLUS commercial sterility detection system and traditional national standard plate counting method were used for detection. After incubation at（36±1）℃ for 7 days simultaneously,the CHARM EPIC-PLUS commercial sterility detection system and the commercial sterility national standard method were used for detection, and the results were compared and analyzed.[Result] The results of the CHARM EPIC-PLUS commercial sterility detection system were highly consistent with the detection results of the commercial sterility national standard detection method. Moreover,compared with the 7-day detection cycle of the traditional commercial sterility national standard detection method,the CHARM EPIC-PLUS commercial sterility detection system significantly shortened the detection time to 48 hours.[Conclusion] The detection results of the CHARM EPIC-PLUS commercial sterility detection system were in good consistency with the national standard detection method of commercial sterility. It is an efficient,accurate detection method with broad application prospects.

Key words: adenosine triphosphate（ATP）bioluminescence, commercial sterility, rapid detection technology