In this paper,the first method of GB 5009.82-2016 RP-HPLC and UV detector were used to detect the vitamin A a content of breast milk in three different periods, and the results were compared.According to the experimental data,the content of vitamin A in breast milk is similar to that in cow's milk during colostrum period, but with the extension of lactation period, the content of vitamin A in breast milk gradually decreases.The detection wavelength was 325 nm,and the peak time of vitamin A in breast milk,milk and reference materials was 3.297 min. The detection limit of vitamin A was 3.98 μ g/100g,and the quantitative limit of vitamin A was 11.9 μ g/100g. Within this range,the linear regression equation y = 1.409 0x-0.002 47, R2 = 0.999 5.
[1] 孙丽,张士发. 芜湖地区622例母乳营养成分检测分析[J]. 右江民族医学院学报,2020,42(6):749-753.
[2] 汪之顼. 母乳成分检测,该不该测?[A]. 见:中国疾病预防控制中心达能营养中心. 达能营养中心2020年论文汇编:孕期营养、母乳喂养[C]. 中国保健营养杂志社,2020.
[3] 丁明,李伟,张玉梅,等. 中国北方地区妇女饮食与母乳中氨基酸组成的相关性分析[A]. 见:营养与慢性病——中国营养学会第七届理事会青年工作委员会第一次学术交流会议论文集[C]. 天津:中国营养学会青年工作委员会:中国营养学会,2010.
[4] 赵晓慧,赵艾,张玉梅,等. 北方某县乳母膳食及母乳中水溶性维生素、矿物质含量的相关性分析[A]. 见:营养与慢性病——中国营养学会第七届理事会青年工作委员会第一次学术交流会议论文集[C]. 天津:中国营养学会青年工作委员会:中国营养学会,2010.
[5] 刘喜红,丁宗一. 母乳库的医学应用[J]. 中国当代儿科杂志,2014,16(7):674-678.
[6] 柏丹丹. 母乳营养成分含量的测定:[硕士论文][D]. 苏州:苏州大学,2013.
[7] 吴建之,苏吉梅,朱燕. 母乳与牛乳中微量元素的比较[J]. 环境污染与防治,2000(1):40-41.
[8] 张兰威,周晓红,肖玲,等. 人乳营养成分及其变化[J]. 营养学报,1997(3):111-114.
