At present, the method for detecting the content of lutein in infant formula milk powder (hereinafter referred to as "infant formula powder") is based on the national food safety standard gb5009.248-2016 determination of lutein in food. Beidahuang Wandashan Dairy Co.,Ltd. has been using high performance liquid chromatography to determine the content of lutein in infant formula powder.During the test of analyzing and quantifying lutein in infant formula powder by high performance liquid chromatography,the uncertainty of standard solution, sample pretreatment,standard curve fitting,instrument repeated injection and other aspects are analyzed and the mathematical model is established,The uncertainty of lutein content in the final synthetic infant formula powder.Results under the 95.0% confidence interval,the expanded uncertainty of lutein content in infant formula powder was 5.62μg / 100g,the content of lutein in infant formula powder was (234.30±5.62)μg/100 g,k=2.Conclusion: the relative uncertainty introduced by the concentration of standard solution is the main factor affecting the uncertainty of lutein content in infant formula powder.