Purpose: In order to accurately determine the content of vitamin D in milk powder and simplify the pretreatment process of national standard method, a method for the determination of vitamin D2 and D3 in milk powder was established. After saponification,n-hexane extraction and concentration,the sample and the standard solution were purified by semi-preparative NP-HPLC,analyzed by RP-HPLC,quantified by external standard method. Result: There was a good linear relationship between vitamin D2 and D3 in the range of 0.01~0.50 μg/mL. The detection limit was 0.7 μg/100 g and the quantitative limit was 2.0 μg/100 g. The results of six determinations of vitamin D2 and D3 were within the range of characteristic values,and the RSD of reproducibility was 1.07% and 2.38%. The recoveries of vitamin D2 and vitamin D3 were 89.50%~98.70% and 88.17%~95.90%. The relative standard deviations were not higher than 8.81%. Conclusion: The determination of vitamin D in a variety of commercial milk powder showed that the simple and accurate method can be provided a basis for the determination of vitamin D2 and D3in milk powder.
LI Yuan, FAN Fangfang, ZHANG Minjuan, ZHANG Lijuan, KANG Jie
. Determination of Vitamin D2 and D3 in Milk Powder by Semi-preparative HPLC[J]. China Dairy, 2022
, 0(3)
: 66
-70
.
DOI: 10.12377/1671-4393.22.03.14
[1] Wagner C L,Greer F R.Prevention of rickets and vitamin D deficiency in infants,children,and adolescents[J]. Pediatrics,2008,122(5):1142-1152.
[2] Lee J M,Smith J R,Philipp B L,et al.Vitamin D deficiency in ahealthy group of mothers and newborn infants[J]. Clinical Pediatrics,2007,46(1):42-44.
[3] 贾宏信. 婴幼儿配方乳粉中脂溶性维生素的稳定性探究[J]. 中国乳品工业,2018,46(3):16-18.
[4] Onwuneme C,Diya B,Uduma O,et al.Correction of vitamin D deficiency in a cohort of newborn infants using daily 200 IU vitamin D supplementation[J]. Irish Journal of Medical Science,2016,185(3):683-687.
[5] Glendenning P,Inderjeeth C A.Screening for vitamin D deficiency:defining vitamin D deficiency,target thresholds of treatment and estimating the benefits of treatment[J]. Pathology,2012,44(2):160-165.
[6] 国家标准局. GB 5009.82—2016 食品安全国家标准食品中维生素A、D、E的测定[S]. 北京:中国标准出版社,2016.
[7] 朱姜,张鹏,杨嘉,等. 高效液相色谱-串联质谱法测定奶粉中的维生素A、维生素D、维生素E[J]. 中国卫生检验杂志,2015,25(11):1733-1736,1739.
[8] 张莹,袁辉,远辉. 高效液相色谱-串联质谱法测定婴幼儿乳粉中维生素D2和D3[J]. 食品工业,2015,36(5):261-264.
[9] 王多娇,颜春荣,徐春祥. 超高效液相色谱-串联质谱法测定食品中维生素D[J]. 安徽农业科学,2020,48(22):190-192,205.
[10] 仲宣惟,夏瑞,施捷. HPLC法测定保健食品中维生素D2、D3的含量[J]. 药物分析杂志,2007,27(11):1773-1774.
[11] 赵玉富,咸伟玥. 二维液相色谱法同时测定饲料中维生素A、维生素D3、维生素E[J].饲料博览,2019(4):51-54.
[12] 许强,刘金生,王轶鹏,等. 反相高效液相色谱法测定维生素D2和维生素D3[J]. 理化检验(化学分册),2011,47(10):1168-1169,1172.
[13] 国家标准局. GB/T 27404—2008 实验室质量控制规范食品理化检测[S]. 北京:中国标准出版社,2008.
