The stability of fat-soluble vitamin D3 in infant formula milk powder（referred to as "infant formula"） during storage was tracked and studied. Test method：after saponifying,extracting,purifying and concentrating 3 kinds of infant formula powders with potassium hydroxide ethanol solution,they were prepared by normal-phase high-performance liquid chromatography column,separated by reverse-phase C18 high-performance liquid chromatography column,and detected by ultraviolet detector and external standard quantitative method. Test results：when the weighing sample of infant formula powder is 10.0 g and the constant volume is 2 mL,the detection limit of the method is 0.303 μg/100g,and the quantification limit of the method is 0.909 μg/100 g. In this range,the linear regression equation Y=3.3608X+0.0388,at this time R2=0.999 1. Conclusion：the content of vitamin D3 in infant formula is less affected by normal temperature and cool and dry environment,the content of fat-soluble vitamin D3 decreases due to temperature decomposition under low and high temperature conditions with prolonged storage time.
WANG Fang, BAO Yihong, LAN Huijuan
. Study on the Stability of Vitamin D3 InInfant Formula Milk Powder under Different Storage Conditions[J]. China Dairy, 2022
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: 65
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DOI: 10.12377/1671-4393.22.07.14
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