[Objective] To understand the growth and decline of dairy cows through immune antibody monitoring. To establish the routine immune procedure for FMD vaccine of dairy cattle in Yanping district,to improve the effective immune protection,to explore the time of producing antibody and the lasting time of immune antibody of two FMD vaccines in Yanping District,to provide scientific basis for prevention of foot-and-mouth disease. [Methods ]A one-off Vacutainer was used for blood sample collection,and liquid-phase blocking Elisa was used for antibody detection. Antibody titers were determined according to the Animal Disease Surveillance Program of the animal husbandry and veterinary authorities. [Subjects] Two large-scale cattle farms in Yanping District, Nanping City, Fujian Province, were selected to purchase vaccines from the government （Zhongmu Industry） and to purchase vaccines from themselves （Jinyu, Inner Mongolia）,each selected a certain number of newborn calves （20 calves and 20 corresponding female）. [Results] The qualified rate of type O antibody was 100% on the day of birth of cow and calf in farm A. After reaching the peak at the fourth week, the qualified rate of type O antibody decreased to 50% at the 12th week. The first immunization time was 12 weeks after birth of calf,the qualified rate of antibody decreased to 55% at the 6th week after the first immunization,and the second immunization was confirmed to be the 6th week after the first immunization,the qualified rate of antibody decreased to 55% in 9 weeks,the first immunization time was 9 weeks after birth,the qualified rate of antibody decreased to 28% in 3 weeks after the first immunization,and the second immunization time was 3 weeks after the first immunization. [Conclusion] According to the results of monitoring test,the immunization program of field a was designed as the first immunization at 12 weeks after birth,the second immunization at 6 weeks after birth,and the third immunization every 4 months the immunization program of field B was designed as first immunization at 9 weeks after birth,second immunization at 3 weeks after birth,and then every 4 months.