[Objective]Clostridium perfringens can infect calves through various ways such as digestive tract and trauma,resulting in diarrhea and sudden death of calves.[Method]In this experiment, field investigation, pathological autopsy and smear staining microscopy of diseased tissues were used to diagnose the cases of calf death caused by suspected Clostridium perfringens infection,and then corresponding comprehensive prevention and control schemes were adopted.[Result]The field investigation showed that the incidence of the disease was 16.35 %（34 /208）,and the mortality rate was 11.76%（4/34）.Finally,30 calves without therapeutic value were eliminated. The results of smear and Wright 's staining microscopy of liver,intestinal contents,mesenteric lymph nodes and other tissues of dead cattle showed that the pathogenic bacteria were Gram-positive,rounded at both ends,with obvious capsule of coarse bacilli.[Conclusion]Based on the field investigation,medical history analysis and morphological observation of bacteria,it was determined that the infection of Clostridium perfringens was the cause of the death of calves. The main route of infection was the digestive tract transmission of feeding cow residues and unfermented wrapped silage.Through emergency immunization,stopping feeding contaminated forage and drinking water disinfection, the further spread of the disease was effectively controlled.
TANG Guochao, ZHANG Yi, JIN Tinghui, QI Yayin, WANG Chenyu, WEI Yong
. Diagnosis and Comprehensive Control of Clostridium Perfringens Infection in Calves[J]. China Dairy, 2022
, 0(11)
: 63
-66
.
DOI: 10.12377/1671-4393.22.11.11
[1] Xiaoting W,Chengcheng N,Chun H J,et al.Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang,China[J]. Journal of Global Antimicrobial Resistance,2021,27:41-45.
[2] Khan M U Z,Humza M,Yang S,etal.Occurrence and toxicogenetic profiling of Clostridium perfringens in buffalo and cattle:An update from Pakistan[J]. Toxins (Basel),2021,13(3):212.
[3] Junior C,Silva R,Lobato F,et al.Gas gangrene in mammals: a review[J]. Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians,Inc,2020,32(2):175-183.
[4] Raymond K,Hall L J.An update on the human and animal enteric pathogen Clostridium perfringens.Emerging Microbes&Infections,2018,7(1):141-156.
[5] Raymond K,Shabhonam C,Sarah A,et al.Probing genomic aspects of the multi-host pathogen clostridium perfringens reveals significant pangenome diversity, and a diverse array of virulence factors[J].Frontiers in Microbiology,2017,8:2485.
[6] Uzal F A,Freedman J C,Shrestha A,et al.Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease[J].Future Microbiolgy,2014,9(3):361-77.
[7] Uzal F A,Mcclane B A,Cheung J K,et alAnimal models to study the pathogenesis of human and animal Clostridium perfringens infections[J].Veterinary Microbiology, 2015,179(1-2):23-33.
[8] Oliveira R D C,Júnior C A D O,Alves G G,et al.Cattle and goats' humoral response to vaccination with Clostridium perfringens type D purified epsilon toxoids[J].Anaerobe,2021,72:102465.
[9] Júnior C A D O,Duarte M C,Assis R,et al.Humoral responses in cattle to commercial vaccines containing Clostridium perfringens epsilon toxoid and C.botulinum types C and D toxoids last less than a-year[J].Anaerobe,2019,59:72-75.
[10] 阮继湘,刘文晓,江波,等.规模化奶牛场犊牛腹泻的病原检测与分析报告[J].中国乳业,2021(9):97-101.
