DAIRY INDUSTRY

Effects of Different Heat Treatment Processes on Five Active Substances in Milk

Expand
  • 1Dairy Nutrition and Function Key Laboratory of Sichuan Province,New Hope Dairy Co.,Ltd.,Chengdu Sichuan 610000;
    2College of Biomass Science and Engineering,Sichuan University,Chengdu Sichuan 610065

Online published: 2023-06-30

Abstract

[Objective]Milk,as a natural source of animal protein with rich nutritional value,is rich in calcium,protein and other nutrients,but also contains rich biological active substances,including immunoglobulin,lactoferrin,α-lactobumin,β-lactoglobulin and lactoperoxidase.[Method]The effects of six different heat treatments with different temperature and time combinations on the active substances in milk were studied.[Result]The content of active substance decreased with the increase of heat treatment intensity.The lactoperoxidase with the worst heat resistance was completely inactivated at 80 ℃/15 s.The highest heat resistance is α-lacubumin,which has a retention rate of 25% even at the highest heat treatment at 137 ℃/4 s.Under the heat treatment intensity of 85 ℃/15 s,the retention rate of α-lactoalbumin is not less than 90%,but the retention rate of lactoferrin and immunoglobulin G is less than 5%,and the lactoperoxidase is almost completely inactivated.The temperature sensitivity of the five active substances was as follows:lactoperoxidase>lactoferrin>immunoglobulin G>β-lactoglobulin>α-lactobumin.[Conclusion]The test results of several active substances in the combined heat treatment emulsion at different temperature and time showed that different heat treatment processes had obvious influence on the retention of active substances.The research results of this paper provide theoretical basis and data support for enterprises to choose the appropriate heat treatment process to produce series of products with different active ingredients.

Cite this article

XIA Zhongyue, QIU Ju, TAN Lianying, SONG Yanmei, QIAN Chenglin, FAN Guangcai, DONG Chenghu, FU Lin . Effects of Different Heat Treatment Processes on Five Active Substances in Milk[J]. China Dairy, 2023 , 0(6) : 89 -94 . DOI: 10.12377/1671-4393.23.06.17

References

[1] 龚婷,王宣敬.牛乳的营养价值及功能特性研究进展[J].甘肃畜牧兽医,2019,49(12):12-15.
[2] 王慧,方洛云,熊本海,等.牛乳生物活性成分及其研究进展[J].中国乳业,2018(8):62-67.
[3] 刘栓,李宜坤,万丹,等.乳铁蛋白生物学功能研究进展[J].动物营养学报,2020,32(4):1508-1515.
[4] 郭善楠,孙宇超.牛乳中免疫球蛋白的生物功能及其分离、纯化研究进展概述[J].中国乳业,2020(12):58-59.
[5] 杜薇滢,李发弟,张养东,等.乳过氧化物酶研究进展[J].食品工业,2018,39(9):236-240.
[6] 程金波,王加启,李珊珊,等.不同热处理方式对牛奶中IgG和乳铁蛋白的影响[J].华北农学报,2010,25(S1):170-174.
[7] 关荣发,贾振宝,黄光荣,等.高效液相色谱法测定牛乳中α-乳白蛋白[J].食品与机械,2011,27(1):60-62.
[8] Luis R D,Pérez M D,Sánchez L,et al.Kinetic and thermodynamic parameters for heat denaturation of Cry1a(b) protein from transgenic maize (Zea mays)[J].Journal of Food Science,2008,73(6):C447-C451.
[9] 花榜清. 牛乳中乳过氧化物酶活性的影响因素[J].乳业科学与技术,2021,44(5):23-26.
[10] 张和平,郭军,李立民,等.免疫乳中IgG热变性动力学研究[J].中国乳品工业,2001,29(4):4-8.
[11] Boitz L I,Fiechter G,Seifried R K,et al.A novel ultra-high performance liquid chromatography method for the rapid determination of β-lactoglobulin as heat load indicator in commercial milk samples[J].Journal of Chromatography A,2015,1386:98-102.
[12] Potonik K,Gantner V,Kuterovac K,et al.Mare’s milk:Composition and protein fraction in comparison with different milk species[J].Mljekarstvo,2011,61(2):107-113.
[13] Sawyer L,Kontopidis G.The core lipocalin bovine β-lactoglobulin[J].Biochimica et Biophysica Acta,2000,1482(1-2):136-148.
[14] Creamer L K, Loveday S M,Sawyer L.Encyclopedia of dairy science[M]. Second Edition. San Diego:Academic Press,2011.
[15] Fox P F,Uniacke-Lowe T,McSweeney P L H,et al.Dairy Chemistry and Biochemistry[M].Second Edition.Switzerland:Springer,2015.
[16] Sakkas L,Moutafi A,Moschopoulou E,et al.Assessment of heat treatment of various types of milk[J].Food Chemistry,2014,159(3):293-301.
[17] Barret N E,Grandison A S,Lewis M J.Contribution of the lactoperoxidase system to the keeping quality of pasteurized milk[J].Journal of Dairy Research,1999,66(1):73-80.
[18] Claeys W L,Cardoen S,Daube G,et al.Raw or heated cow milk consumption:Review of risks and benefits[J].Food Control,2013,31(1):251-262.
Outlines

/