[Objective] The hydrolysate of whey protein powder contains α- amino small molecule substances of the content in product is relatively high. Find a detection method to detect the content of this part of the substance,and report the proportion of protein as a reference value for the degree of hydrolysis.[Method] Kjeldahl nitrogen determination method was used to detect total protein content,while using ninhydrin and α- amino groups take color reaction,read the absorbance value of the reaction solution,and substitute it into the working curve to obtain the content of α- amino small molecule substances of the ratio amino to total protein is the content （in protein）.[Result] α- amino of small molecule content （in protein） standard deviation results is 1.91,with a relative labeling deviation of 3.5%. The method has good repeatability and the recovery rate also meets the requirements for spiked recovery. [Conclusion] Ninhydrin colorimetric method for detecting the presence of α- amino small molecule substances have obvious advantages and can be used in combination with Kjeldahl method to monitor the protein hydrolysis effect.
ZHANG Xiao
,
ZHAO Ziqi
,
ZHAO Yanjie
,
GU Yaning
,
ZHANG Daoyun
,
CAO Wenyan
,
TIAN Guiyan
,
LIU Qingshuang
,
CHEN Juan
,
BAO Gangxue
,
WANG Ze
,
MA Jianjun
,
LU Gang
. Determination of the Proportion of Small Molecule Substances Containing α- amino Groups in Total Protein Content of Hydrolyzed Whey Protein Powder[J]. China Dairy, 2023
, 0(10)
: 82
-87
.
DOI: 10.12377/1671-4393.23.10.15
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