[Objective] The detection method of protease activity in polyglucose and stevioside glycosides was established. [Method] Single factor experiment and orthogonal experiment were used to optimize the assay conditions of protease activity,and the accuracy of the recovery method was investigated. [Result] The optimal conditions for the detection of protease activity in polyglucose and stevioside glycosides were to dissolve samples with boric acid buffer solution,sample size to be tested was 5 g,reaction time was 20 min,reaction temperature was 40 ℃,pH value was 10.5,and filtrate standing time was 20 min.The experimental results showed that the accuracy of the method was 64%~102% and the relative standard deviation was less than 1.3%. [Conclusion] This method was suitable for the detection of protease content in stevioside polyglucose after comprehensive verification of curve correlation and accuracy.
ZHANG Lin, YU Min, LIU Yanming, ZHANG Yahua, CHEN Juan, LU Gang
. Study on the Method of Detecting Protease Activity in Polyglucose and Stevia Glycosides[J]. China Dairy, 2024
, 0(11)
: 149
-156
.
DOI: 10.12377/1671-4393.24.11.24
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