[Objective] In August 2023,an outbreak of infectious disease characterized by respiratory symptoms was reported on one cattle farm in Tianjin. To determine the cause of the disease,we conducted etiological diagnosis of the relevant pathogens in the affected calves. [Method] Lung specimens were collected from dead calves. We used the PCR method to detect seven bovine respiratory pathogens,including Bovine parainfluenza virus type 3,Bovine respiratory syncytial virus,Bovine herpesvirus type 1,Bovine adenovirus type 3,Mycobacterium bovis, Bovine Klebsiella bovis and Mycoplasma bovis. [Result] The results showed that two pathogens,BHV-1 and BAdV-3,were detected in the samples,and there was mixed infection. No other pathogens were detected. [Conclusion] This study confirmed the existence and prevalence of BHV-1 and BAdV-3 in dairy cows in this cattle farm,which provided reference for the prevention and control of respiratory diseases.
YUAN Wenhuan
,
CHEN Jingyi
,
ZHANG Tianqi
,
JIA Chuntao
. A Case of Etiological Diagnosis of Bovine Respiratory Disease[J]. China Dairy, 2025
, 0(1)
: 41
-45
.
DOI: 10.12377/1671-4393.25.01.08
[1] Baptista A L,Rezende A L,de Almeida Fonseca P,et al.Bovine respiratory disease complex associated mortality and morbidity rates in feedlot cattle from southeastern Brazil[J].The Journal of Infection in Developing Countries,2017,11(10):791-799.
[2] 刘志鹏,董秀梅,师东方,等.牛呼吸道疾病综合征的主要病原[J].畜牧兽医科技信息,2012(12):9-11.
[3] Narita M,Yamada M,Tsuboi T,et al.Immunohistopathology of calf pneumonia induced by endobronchial inoculation with bovine adenovirus 3[J].Veterinary Pathology,2002,39(5):565-571.
[4] Yamada M,Narita M,Nakamura K,et al.Apoptosis in calf pneumonia induced by endobronchial inoculation with bovine adenovirus type 3(BAV-3)[J].Journal of Comparative Pathology,2003,128(2/3):140-145.
[5] 何姝凡,岳华,汤承,等.牛腺病毒3型的研究进展[J].畜牧兽医学报,2022,53(4):1030-1040.
[6] Harrison S C.Principles of virus structure[J].Fields Virology,2001,1:53-85.
[7] Mayfield J E,Good P J,Vanoort H J,et al.Cloning and cleavage site mapping of DNA from bovine herpesvirus 1(cooper strain)[J].Journal of Virology,1983,47:259-264.
[8] Muylkens B,Thiry J,Kirten P,et al.Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis[J].Veterinary Research,2007,38(2):181-209.
[9] 郭长梅. 常见牛呼吸道疾病病因分析与综合防控探讨[J].中国乳业,2024(7):64-68.
