[Objective] This study aimed to evaluate the dynamic effects of direct and indirect sterilization processes on the content and composition of free monosaccharides in bovine milk,addressing the current research gap in comparing these two heating methods.[Method] The contents of arabinose,galactose,mannose,ribose,xylose,glucose,and fucose were quantitatively analyzed using liquid chromatography-mass spectrometry （LC-MS）. Statistical evaluation was performed via one-way ANOVA,Tukey's post-hoc test,principal component analysis （PCA）,and hierarchical clustering for multivariate analysis.[Result] Heat treatment significantly altered the monosaccharide profile：the contents of galactose,mannose,ribose,and fucose decreased in a dose-dependent manner with increasing temperature （all p<0.01）. Glucose content increased significantly by 75.0% [（37 182.40±1 063.18）ng/mL] at 75 ℃ but decreased by approximately 8.2% and 7.3% at 122 ℃ and 130 ℃,respectively. Hierarchical clustering analysis further confirmed the dominant role of temperature in regulating monosaccharides,showing higher similarity between the 130 ℃ and 75 ℃ groups,while the 122 ℃ group was distinctly separated. This indicates that short-time high-temperature treatment can inhibit the accumulation of early Maillard reaction products and reduce excessive degradation of heat-sensitive monosaccharides.[Conclusion] This study was the first to reveal the effects of direct and indirect sterilization processes on the free monosaccharide content in raw bovine milk. It provided new experimental data for understanding the mechanisms of lactose degradation and monosaccharide transformation during thermal processing and offers a scientific basis for optimizing dairy processing technologies.
QI Yingjie
,
XIAO Ran
,
DU Shuang
,
ZHANG Hongyan
,
ZHANG Yangdong
,
ZHENG Nan
,
ZHAO Yankun
,
WANG Jiaqi
,
NIU Tianjiao
. Effects of Different Heat Treatments on the Content of Free Monosaccharides in Bovine Milk[J]. China Dairy, 2025
, 0(11)
: 7
-14
.
DOI: 10.12377/1671-4393.25.11.02
[1] Fox P F.Milk:An overview[J]. Milk proteins,2008:1-54.
[2] Oftedal O T.The evolution of milk secretion and its ancient origins[J]. Animal,2012,6(3):355-368.
[3] Renzone G,Arena S,Salzano A M,et al.Proteomic characterization of nonenzymatic modifications induced in bovine milk following thermal treatments[M]. Proteomics in Food Science,2017:241-260.
[4] Newburg D S,Neubauer S H.Carbohydrates in milk[J]. Handbook of milk composition,1995:349-273.
[5] McSweeney P,Fox P. Advanced Dairy Chemistry:Volume 3:Lactose,Water,Salts and Minor Constituents[M]. Cham:Springer Nature,2022.
[6] Englyst K N,Englyst H N.Carbohydrate bioavailability[J]. British Journal of Nutrition,2005,94(1):1-11.
[7] Szilagyi A.Digestion,absorption,metabolism,and physiological effects of lactose[M]. Lactose. Elsevier. 2019:49-111.
[8] Chaturvedi P,Warren C D,Altaye M,et al.Fucosylated human milk oligosaccharides vary between individuals and over the course of lactation[J]. Glycobiology,2001,11(5):365-372.
[9] Ruiz-Moyano S,Totten S M,Garrido D A,et al.Variation in consumption of human milk oligosaccharides by infant gut-associated strains of Bifidobacterium breve[J]. Applied and environmental microbiology,2013,79(19):6040-6049.
[10] Chandel N S.Carbohydrate metabolism[J]. Cold Spring Harbor Perspectives in Biology,2021,13(1):a040568.
[11] Singh P,Gandhi N.Milk preservatives and adulterants:processing,regulatory and safety issues[J]. Food Reviews International,2015,31(3):236-261.
[12] Xu B,Li S,Ding W,et al.From structure to function:A comprehensive overview of polysaccharide roles and applications[J]. Food Frontiers,2025,6(1):15-39.
[13] El Khadem H.Carbohydrate chemistry:monosaccharides and their oligomers[M]. Amsterdam:Elsevier,2012.
[14] Chaudhary S,Jain V P,Jaiswar G.The composition of polysaccharides:monosaccharides and binding,group decorating,polysaccharides chains[M]//Innovation in nano-polysaccharides for eco-sustainability. Elsevier,2022:83-118.
[15] Nuño J C,Sánchez-Valdenebro I,Pérez-Iratxeta C,et al.Network organization of cell metabolism:monosaccharide interconversion[J]. Biochemical Journal,1997,324(1):103-111.
[16] Khowala S,Verma D,Banik S P.BIOMOLECULES:(INTRODUCTION,STRUCTURE & FUNCTION)[J]. Indian Institute of Chemical Biology,2008:3-92.
[17] Chandel N S.Glycolysis[J]. Cold Spring Harbor Perspectives in Biology,2021,13(5):a040535.
[18] Tzia C,Giannou V,Lebesi D,et al.Chemistry and functional properties of carbohydrates and sugars (monosaccharides,disaccharides,and polysaccharides)[J]. Sweeteners. Nutritional aspects,applications,and production technology,2012:11-45.
[19] Neves L N O,de Oliveira M A L. Effects of enzymatic lactose hydrolysis on thermal markers in lactose-free UHT milk[J]. Journal of Food Science and Technology,2020,57(9):3518-3524.
[20] Bai G,Cheng L,Peng L,et al.Effects of ultra‐high‐temperature processes on metabolite changes in milk[J]. Food Science & Nutrition,2023,11(6):3601-3615.
[21] Jensen S,Jansson T,Eggers N,et al.Storage-induced changes in the sensory characteristics and volatiles of conventional and lactose-hydrolyzed UHT processed milk[J]. European Food Research and Technology,2015,240(6):1247-1257.
