CHINA EXCELLENT MILK PROJECT

Effects of Heat Treatment on Dairy Flavor and the Degree of Maillard Reaction

  • CHEN Yinge ,
  • WEN Fengliang ,
  • XIAO Ran ,
  • ZHANG Yangdong ,
  • ZHENG Nan ,
  • ZHAO Yankun ,
  • ZHANG Hongyan ,
  • OU Guobing ,
  • WANG Jiaqi ,
  • NIU Tianjiao
Expand
  • 1 Beijing Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences,National Market Supervision Administration Key Laboratory (Digital Intelligent Monitoring Technology for Dairy Quality),Beijing 100193;
    2 Mengniu Fresh Milk Products(Tianjin)Co.,Ltd.,Tianjin 301700;
    3 Inner Mongolia Mengniu Dairy(Group)Co.,Ltd.,Hohhot Inner Mongolia 011500;
    4 Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091;
    5 Xinjiang Xiyuchun Dairy Co.,Ltd.,Changji Xinjiang 831201

Online published: 2025-12-22

Abstract

[Objective] This study systematically investigated the effects of different heat treatment conditions on milk flavor and the degree of the Maillard reaction. [Method] Raw milk,10 indirect sterilization processes (including three holding times:2 s,15 s,30 min and ten temperatures:63~135 °C),and a direct sterilization process (130 °C/0.5 s) were established. The effects of heat treatment intensity on dairy product quality were comprehensively evaluated using electronic nose flavor analysis,furosine,and lactulose content determination. [Result] The electronic nose effectively distinguished the flavor profiles of different heat treatment groups. The direct sterilization process showed significant flavor differences compared to other groups. Notable flavor differences were also observed between the (72~90 °C)/15 s and (95~122 °C)/15 s indirect sterilization processes,with the main volatile differential compounds being sulfides,aromatic components,and nitrogen oxides. The contents of furosine and lactulose increased with rising temperature,showing a sharp rise particularly at 135 °C/15 s,indicating severe heat damage. In contrast,the direct sterilization process (130 °C/0.5 s) effectively inhibited the formation of Maillard reaction products,with furosine and lactulose contents falling between those of the 90~95 °C/15 s and 72~80 °C/15 s processes,respectively. [Conclusion] The direct sterilization process achieves sterilization and extends shelf life while significantly controlling heat damage and retaining favorable flavor,making it suitable for producing high-quality UHT milk. Low-temperature indirect sterilization processes,with low thermal load and minimal flavor changes,are suitable for pasteurized milk. Medium-temperature processes are applicable for extended shelf life (ESL) dairy products. This research provides a theoretical basis and technical support for optimizing dairy heat treatment processes to balance sterilization effectiveness with flavor quality.

Cite this article

CHEN Yinge , WEN Fengliang , XIAO Ran , ZHANG Yangdong , ZHENG Nan , ZHAO Yankun , ZHANG Hongyan , OU Guobing , WANG Jiaqi , NIU Tianjiao . Effects of Heat Treatment on Dairy Flavor and the Degree of Maillard Reaction[J]. China Dairy, 2025 , 0(11) : 42 -50 . DOI: 10.12377/1671-4393.25.11.05

References

[1] Pereira P C.Milk nutritional composition and its role in human health[J]. Nutrition,2014,30(6):619-627.
[2] Haug A,Høstmark A T,Harstad O M.Bovine milk in human nutrition--a review[J]. Lipids in Health and Disease,2007,6:25.
[3] Mills S,Ross R P,Hill C,et al.Milk intelligence:Mining milk for bioactive substances associated with human health[J]. International Dairy Journal,2011,21(6):377-401.
[4] Politis IChronopoulou R.Milk peptides and immune response in the neonate[J]. Advances in Experimental Medicine and Biology,2008,606:253-269.
[5] Fiat A-M,Migliore-Samour D,Jollès P,et al.Biologically active peptides from milk proteins with emphasis on two examples concerning antithrombotic and immunomodulating activities[J]. Journal of Dairy Science,1993,76(1):301-310.
[6] Zimecki M, Kruzel M L.Milk-derived proteins and peptides of potential therapeutic and nutritive value[J]. Journal of Experimental Therapeutics & Oncology,2007,6(2):89-106.
[7] González-Chávez S A,Arévalo-Gallegos S,Rascón-Cruz Q. Lactoferrin:structure,function and applications[J]. International Journal of Antimicrobial Agents,2009,33(4):301.
[8] Månsson H L.Fatty acids in bovine milk fat[J]. Food and Nutrition Research,2008,52:1-6.
[9] Benjamin S, Spener F.Conjugated linoleic acids as functional food:an insight into their health benefits[J]. Nutrition & Metabolism,2009,6:1-36.
[10] Gaucheron F.Milk and dairy products:a unique micronutrient combination[J]. Journal of the American College of Nutrition,2011,30(5 Suppl 1):400-409.
[11] Su X,Tortorice M,Ryo S,et al.Sensory lexicons and formation pathways of off-aromas in dairy ingredients:A review[J]. Molecules,2020,25(3):1-8.
[12] Cadwallader K,Rsingh T.Flavours and off-flavours in milk and dairy products[J]. Advanced Dairy Chemistry,2009,1(1):631-690.
[13] Bassette R,Fung D Y,Mantha V R.Off-flavors in milk[J]. Critical Reviews in Food Science and Nutrition,1986,24(1):1-52.
[14] Carunchia Whetstine M E,Croissant A E,Drake M A. Characterization of dried whey protein concentrate and isolate flavor[J]. Journal of Dairy Science,2005,88(11):3826-3839.
[15] Whetstine M E C, Drake M A. The flavor and flavor stability of skim and whole milk powders[J]. American Chemical Society, 2007,9:217-251.
[16] Jo Y,Benoist D M,Barbano D M,et al.Flavor and flavor chemistry differences among milks processed by high-temperature,short-time pasteurization or ultra-pasteurization[J]. Journal of dairy science,2018,101(5):3812-3828.
[17] Al-Attabi Z,D’Arcy B R,Deeth H C. Volatile sulfur compounds in pasteurised and UHT milk during storage[J]. Dairy Science & Technology,2014,94(3):241-253.
[18] Smith T J,Campbell R E,Jo Y,et al.Flavor and stability of milk proteins[J]. Journal of Dairy Science,2016,99(6):4325-4346.
[19] Adhikari A,KSingal O P.Effect of dissolved oxigen content on the flavour profile of UHT milk during storage[J]. The International Journal of Dairy Technology,1992,47:6.
[20] 全国畜牧业标准化技术委员会. 巴氏杀菌乳和UHT灭菌乳中复原乳的鉴定:NY/T 939-2016[S].
[21] Donato L,Guyomarc’h F,Amiot S,et al.Formation of whey protein/κ-casein complexes in heated milk:Preferential reaction of whey protein with κ-casein in the casein micelles[J]. International Dairy Journal,2007,17(10):1161-1167.
[22] Van Boekel M A J S. Effect of heating on Maillard reactions in milk[J]. Food Chemistry,1998,62(4):403-414.
[23] Wang Y,Guo M,Wu P,et al.Size-dependent composition and in-situ structure analysis of the milk fat globule membrane in buffalo milk[J]. Food Chemistry,2025,464:141766.
[24] Al-Attabi Z,D’Arcy B R,Deeth H C. Volatile sulphur compounds in UHT milk[J]. Critical Reviews in Food Science and Nutrition,2009,49(1):28-47.
[25] Rudy D W,Nieuwenhuijse H.Kinetic modelling of the formation of sulphur-containing flavour components during heat-treatment of milk[J]. International Dairy Journal,2008,18(5):539-547.
[26] Lynch J M,Lock A L,Dwyer D A,et al.Flavor and stability of pasteurized milk with elevated levels of conjugated linoleic acid and vaccenic acid[J]. Journal of Dairy Science,2005,88(2):489-498.
[27] Czerwonka M,Pietrzak S R,Bobrowska K B.Evaluation of 5-hydroxymethylfurfural content in market milk products[J]. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment,2020,37(7):1135-1144.
[28] Baptista J A B,Carvalho R C B. Indirect determination of Amadori compounds in milk-based products by HPLC/ELSD/UV as an index of protein deterioration[J]. Food Research International,2004,37(8):739-747.
[29] Boitz L I,Mayer H K.Evaluation of furosine,lactulose and acid-soluble β-lactoglobulin as time temperature integrators for whipping cream samples at retail in Austria[J]. International Dairy Journal,2015,50:24-31.
[30] Guo Y,Li H,Zhao X,et al.Changes in Maillard reaction products,volatile substances and active proteins of goat milk under different heat treatments[J]. International Dairy Journal,2023,146:105714.
[31] Elliott A J,Datta N,Amenu B,et al.Heat-induced and other chemical changes in commercial UHT milks[J]. Journal Of Dairy Research,2005,72(4):442-446.
[32] Neves L N D O,Marques R,da Silva P H F,et al. Lactulose determination in UHT milk by CZE-UV with indirect detection[J]. Food Chemistry,2018,258:337-342.
Outlines

/