[Objective] In the quality control system of the dairy industry,non-destructive testing technology for sterilized milk has gained increasing attention due to its advantages of low cost and high efficiency.This study focuses on the accuracy of test results of sterilized milk stored at different temperatures when detected under the same temperature calibration module of the RS-6 dairy package shaker.[Method] A certain quantity of sterilized milk was incubated at different temperatures (5,10,15,20,25,30,35,and 40 ℃) and tested using the shaking module calibrated at 20,25,and 30 ℃,respectively.[Result] The experimental results showed that when the temperature difference between the calibration sample during calibration and the tested sample was within ±5 ℃,the accuracy of the detection results from the shaker was the highest, with a pass rate of 100%.However,when the temperature difference exceeded 5 ℃,the detection pass rate significantly decreased as the temperature difference increased.[Conclusion] This research finding holds significant practical importance for optimizing the quality control process of sterilized milk.It can provide dairy enterprises with a more precise and efficient testing method,thereby contributing to the overall improvement of quality management standards in the industry.