[Objective] To establish a post-column derivatization cation exchange chromatography method for determining the contents of asparagine and glutamine in foods for special medical purposes.[Method] The samples were enzymatically hydrolyzed with Streptomyces griseus protease,and the supernatant was collected after centrifugation.It was then separated on a cation exchange chromatography column,derivatized with ninhydrin.The absorbance was detected at 570 nm,and the quantitative analysis was performed using the external standard method.[Result] Asparagine and glutamine exhibited good linearity in the range of 10-250 μmol/L（R²>0.9999）.The limits of quantification was 1.4 μmol/L and 1.1 μmol/L,respectively,the recovery rate was 96.5%-101.1%,and the RSD was 1.0%-2.2%.[Conclusion] This method demonstrated good repeatability and high accuracy,overcoming the drawbacks of complicated operations and poor repeatability associated with pre-column derivatization.It can serve as a complementary approach to the national standard methods,providing technical support for quality control of foods for special medical purposes and government supervision.
QIN Juxiang
,
HUANG Hui
,
QIAN Fei
,
CAI Xia
,
FENG Yinjie
,
ZHANG Zhanlin
,
ZHOU Xiaoqing
. Determination of Asparagine and Glutamine in Foods for Special Medical Purposes by Enzymatic Hydrolysis with Post-Column Derivatization Cation Exchange Chromatography[J]. China Dairy, 2026
, 0(8)
: 129
-134
.
DOI: 10.12377/1671-4393.26.08.18
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