China Dairy ›› 2022, Vol. 0 ›› Issue (1): 58-62.doi: 10.12377/1671-4393.22.01.11

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Isolation,Identification and Drug Sensitivity Test of Pathogens Causing Mastitis in 3 Large-scale Dairy Farms in Xinjiang

CHEN Jie1, WEI Yong2, WANG Chenyu1, CAO Mengyuan1, XIE Jingang2, QI Yayin1,*   

  1. 1College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832000;2Xinjiang Tian’ao Animal Husbandry Co.,LTD.,Kuitun Xinjiang 833200;
  • Online:2022-01-25 Published:2022-02-22

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Abstract: Dairy mastitis is one of the most common diseases in dairy cattle,which brings huge economic losses to dairy farming industry. To investigate the incidence and prevalence of mastitis of dairy cows in large-scale dairy farms in Xinjiang,the field epidemiological investigation and laboratory examination,pathogen isolation,identification and drug resistance analysis were carried out on clinical mastitis cattle from 3 large-scale dairy farms in Xinjiang. A total of 243 milk samples with somatic cell count ≥5 million /mL were collected,and 231 suspected gram-negative enterobacter were isolated by continuous streak culture on Mcconkay agar plate and Eosin-Meilan agar plate,combined with smear,staining and microscopic examination. Escherichia coli was identified as the main pathogen causing clinical mastitis of dairy cows in these three large-scale dairy farms. Results：187 strains of Escherichia coli were identified by PCR,and the isolation rate was 77%,drug sensitivity results showed that Escherichia coli was highly sensitive to cefotaxime,streptomycin,gentamicin,amikacin and ciprofloxacin.

Key words: mastitis, Escherichia coli, isolation and identification, drug sensitive test

CLC Number: 

  • S858.23
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