China Dairy ›› 2022, Vol. 0 ›› Issue (1): 69-72.doi: 10.12377/1671-4393.22.01.13

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Study on the Influence of Relative Liquid Chromatography on Measuring Melamine in Dairy Products

CHEN Maojiao, DENG Yanqin, HE Weiwei, LAN Ning, ZHANG Hongmei   

  1. Newhope Dairy Co.,Ltd.,Sichuan Meishan 620360
  • Online:2022-01-25 Published:2022-02-22

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Abstract: The most commonly used method to detect melamine content in dairy products is high performance liquid chromatography（HPLC）in GB/T22388—2008 method for detecting melamine in raw milk and dairy products. When using high performance liquid chromatography for analysis,the type and proportion of mobile phase have great influence on the test results,especially on the peak time,peak area,peak type and recovery rate. Therefore,it is extremely important to configure the appropriate mobile phase ratio for obtaining good atlas and results. In this paper,the ratio of ions to reagents and acetonitrile in mobile phase was taken as variable,and the influence of the ratio of 85:15,88:12,90:10,92:8 and 95:5 on the test results was explored,and the most suitable ratio of mobile phase in the current laboratory environment was found out,so as to improve the reliability of the test results.

Key words: dairy product inspection, melamine inspection, liquid chromatography, mobile phase, peak effect

CLC Number: 

  • 0657.72
[1] 张庆合,李秀琴,杨总,等. 影响HPLC-IDMS测量准确度因素探讨[A]. 见:中国化学会、国家自然科学基金委员会.中国化学会首届全国质谱分析学术研讨会会议论文集[C].中国化学会、国家自然科学基金委员会:中国化学会,2014.
[2] 袁园. 高效液相色谱法测定乳制品三聚氰胺的含量研究[J].轻工科技,2021,37(9):15-16.
[3] 高丽婷.动物源食品中三聚氰胺残留量检测方法改进研究[J].食品安全导刊,2020(6):98,100.
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[4] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 67 -72 .
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