乳制品检验液相色谱分析方法的建立与优化

doi:10.12377/1671-4393.22.04.14

Abstract

Abstract: High performance liquid chromatography （HPLC） has been widely used in the detection of food additives,veterinary pesticide residues,active nutrients and other fields due to its advantages of high accuracy,good repeatability and simultaneous separation of several or even more than a dozen substances. Reverse phase chromatography is the most common method of liquid chromatography because of its many adjustable parameters, flexible preparation of mobile phase and wide applicability. In the actual inspection work,it is essential to establish suitable analytical methods and equip suitable chromatographic conditions for different target substances. Taking the establishment of reverse phase chromatography as an example, this paper discussed in detail how to establish a liquid chromatography method for a certain component and optimize the method by adjusting the type of mobile phase,column parameters and other conditions,which have certain reference value for the optimization and establishment of the method in the actual dairy product inspection.

Key words: dairy inspection, liquid chromatograph, reversed-phase chromatography, method optimization

CLC Number:

O657.72

CHEN Baixi, SUN Jiahao, LIU Wei, ZHANG Wenqi, WU Xiaohui. Establishment and Optimization of Liquid Chromatography Analysis Method for Dairy Products Inspection[J].China Dairy, 2022, 0(4): 72-76.

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Establishment and Optimization of Liquid Chromatography Analysis Method for Dairy Products Inspection

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