China Dairy ›› 2024, Vol. 0 ›› Issue (11): 149-156.doi: 10.12377/1671-4393.24.11.24

• DAIRY INDUSTRY • Previous Articles     Next Articles

Study on the Method of Detecting Protease Activity in Polyglucose and Stevia Glycosides

ZHANG Lin1, YU Min1, LIU Yanming2, ZHANG Yahua1, CHEN Juan2, LU Gang2,*   

  1. 1Inner Mongolia Mengniu High-Tech Dairy, Co., Ltd., Hohhot Inner Mongolia 011500;
    2Inner Mongolia Mengniu Dairy(Group) Co., Ltd., Hohhot Inner Mongolia 011500
  • Online:2024-11-25 Published:2024-12-10

Abstract: [Objective] The detection method of protease activity in polyglucose and stevioside glycosides was established. [Method] Single factor experiment and orthogonal experiment were used to optimize the assay conditions of protease activity,and the accuracy of the recovery method was investigated. [Result] The optimal conditions for the detection of protease activity in polyglucose and stevioside glycosides were to dissolve samples with boric acid buffer solution,sample size to be tested was 5 g,reaction time was 20 min,reaction temperature was 40 ℃,pH value was 10.5,and filtrate standing time was 20 min.The experimental results showed that the accuracy of the method was 64%~102% and the relative standard deviation was less than 1.3%. [Conclusion] This method was suitable for the detection of protease content in stevioside polyglucose after comprehensive verification of curve correlation and accuracy.

Key words: polyglucose, stevioside, proteaseactivity

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