China Dairy ›› 2026, Vol. 0 ›› Issue (5): 71-78.doi: 10.12377/1671-4393.26.05.11

• DISEASE PREVENTION • Previous Articles     Next Articles

Clinical Efficacy of the Chinese Medicine Compound Rulikang in the Treatment of Bovine Mastitis

TAO Ziqi1, Arsebek Jumamamat1, LI Yali2, GONG Chao1, WANG Bo1, LUO Wanhe3, Omar Yakup1, HAO Zhihui1,4, SUN Yan5, HAN Juncheng1,*, DAI Xiaohua1,*   

  1. 1. Xinjiang Key Laboratory of New Drug Research and Development for Herbivorous AnimaIs (XJ-LNDRDHA),College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi Xinjiang 830052;
    2. College of Animal Science and Technology,Xinjiang Agricultural Vocational and Technical University,Changji Xinjiang 831100;
    3. College of Animal Science and Technology,Tarim University,Animal Disease Diagnosis,Prevention and Control Engineering Laboratory,Alar Xinjiang 843300;
    4. College of Veterinary Medicine,China Agricultural University,Beijing 100193;
    5. Xinjiang Nongmu Investment Group Haozi Animal Husbandry Co.,Ltd.,Changji Xinjiang 831100
  • Online:2026-05-25 Published:2026-06-18

PDF (PC)

1

Abstract: [Objective] This study aimed to verify the antibacterial effect and clinical efficacy of Chinese Medicine Compound Rulikang against pathogenic bacteria of bovine mastitis. [Method] Chinese Medicine Compound Rulikang, consisting of Taraxacum mongolicum, Forsythia suspensa, Akebia quinata, Vaccaria segetalis and Astragalus membranaceus, was prepared into liquid with a crude drug concentration of 1 g/mL by water decoction and concentration. Escherichia coli, Staphylococcus aureus and Streptococcus agalactiae isolated from clinical bovine mastitis cases were used to assess its in vitro antibacterial activity. Thirty bovines suffering from clinical mastitis were assigned into low, medium and high dose groups, while 10 healthy bovines were set as the control group. Intramammary perfusion was performed twice a day for 7 consecutive days. The clinical efficacy and variations of milk protein indicators were observed. [Result] Chinese Medicine Compound Rulikang exhibited strong inhibitory effects on the three pathogenic strains. After 7 days of treatment, the effective rate and cure rate of the high dose group reached 88.2% (15/17) and 82.4% (14/17), respectively. Milk yield of all administration groups was higher than that of the control group. The levels of SA, ALT, AST and LDH in cow whey decreased significantly (P<0.05), whereas lactose content barely changed. [Conclusion] Chinese Medicine Compound Rulikang had prominent therapeutic effects on clinical bovine mastitis, which provided a safe and effective herbal preparation for the disease treatment.

Key words: Chinese Medicine Compound Rulikang, bovine mastitis, in vitro bacteriostasis, clinical efficacy

[1] Na S,Intanon M,Srithanasuwan A,et al.Evidence of vancomycin-resistant Staphylococcus aureus,multidrug-resistant S. aureus,and Enterococcus faecium-causing mastitis in Thailand and Cambodia[J].Veterinary World,2025,18(1):202-209.
[2] 刘昊. 瓜蒌散组分复方灌注剂对奶牛乳房炎致病菌的体外抑菌效果及临床疗效研究[J].中国奶牛,2025(5):28-31.
[3] Maalaoui A,Trimeche A,Marnet P G.Alternative approaches to antibiotics in the control of mastitis in dairy cows:A review[J].Veterinary Research Communications,2025,49(3):150.
[4] 申纪饶. 奶牛乳腺炎细菌分离鉴定及芍药苷对溶血葡萄球菌侵染bMEC抑制作用研究[D].北京:中国农业科学院,2022.
[5] 吕玉霞,杜玉芹,杨俊翠.中药复方灌注剂对奶牛乳房炎致病菌的体外抑菌效果及临床疗效[J].中国乳业,2024(12):86-91.
[6] 刘昊阳,徐媛媛,李思,等.中医对奶牛乳痈的辨证论治及蒲公英的防治研究进展[J].动物医学进展,2022,43(11):108-113.
[7] 韩志帅. 治疗奶牛乳房炎中药灌注剂的研制及其安全性与临床疗效研究[D].河北:河北农业大学,2013.
[8] 孟庆娟. 中药复方灌注剂对奶牛乳房炎的防治效果及作用机理的研究[D].安徽:安徽农业大学,2011.
[9] Ye D Y,Sun J,Li Y Q.Evaluation of toxicity,bacteriostatic,analgesic,anti-inflammatory,and antipyretic activities of Huangqin-Honghua-Pugongying-Jinyinhua extract[J].Veterinary Sciences,2021,8(12):330.
[10] 战永波. 治疗奶牛乳房炎蒙药复方的筛选及其抗炎免疫机理的研究[D].呼和浩特:内蒙古农业大学,2010.
[11] 刘伯承,周望平,杨俊,等.中药复方制剂对奶牛乳房炎主要病原菌体外抑菌活性及治疗效果评价[J].中国奶牛,2024(8):23-28.
[12] 王留,夏晴,付赛赛.复方蒲公英奶牛乳房灌注剂药效学试验研究[J].畜牧产业,2024(4):68-73.
[13] 鲁俊廷. 28 种中药及其复方对奶牛乳房炎病原菌金黄色葡萄球菌的体外抑菌试验[J].中国奶牛,2024(7):46-50.
[14] 皮真,吴秋云,黄琳,等.奶牛乳房炎辨证论治及现代药理作用研究[J].中兽医医药杂志,2018,37(2):34-38.
[15] 闫广伟. 山东省部分地区奶牛场奶杯内衬中分离细菌的多样性和耐药性调查[D].青岛:山东农业大学,2023.
[16] 田荣芳. 中药复方制剂对奶牛隐性乳房炎的治疗效果研究[J].中兽医学杂志,2018(4):104-105.
[17] 王留,方磊涵,郭全海,等.复方蒲公英乳房灌注剂对奶牛临床型乳房炎的防治效果试验[J].山东畜牧兽医,2024,45(3):3-5.
[18] 杨瑞,张春杰,王晓杰,等.中药复方灌注剂对奶牛乳房炎致病菌的体外抑菌效果及临床疗效[J].中国奶牛,2024(3):25-28.
[19] 张宇航,郝景锋,李静姬,等.不同中草药方剂对奶牛乳房炎致病菌体外抑菌试验及临床疗效比较[J].黑龙江畜牧兽医,2017(18):171-173.
[20] 詹经纬,童津津,熊本海,等.奶牛乳腺健康与乳中体细胞数之间关系的研究进展[J].动物营养学报,2021,33(3):1299-1305.
[21] 毛亚洁. 复方中药制剂乳炎清治疗临床型奶牛乳房炎临床效果观察[J].中国草食动物科学,2017,37(2):37-40.
[22] 田琛. 黄芪中药组合方剂对奶牛乳房炎的治疗效果[J].中兽医学杂志,2021(7):8-9.
[23] 孟庆娟,李志明,冯士彬,等.中药复方灌注剂对奶牛乳房炎的防治效果及免疫增强作用[J].中国畜牧兽医,2011,38(7):219-224.
[24] 槐以啟,年士艳,冯磊.血清淀粉样蛋白A的临床运用新进展[J].分子诊断与治疗杂志,2022,14(6):903-906.
[25] 田猛,李纯锦,易康乐,等.复方发酵中草药对奶牛隐性乳腺炎的预防和治疗[J].黑龙江动物繁殖,2020,28(1):4-8.
[26] 郝景锋,李静姬,尹柏双,等.中药复方对奶牛隐性乳房炎血清中LDH、NAG活性的影响[J].黑龙江畜牧兽医,2017(24):134-136.
[27] 白东东,李新圃,杨峰,等.乳黄消口服液治疗奶牛乳房炎的临床疗效观察[J].中国兽医杂志,2021,57(1),87-91,96.
[28] 李睿文,李铁拴,关鸣,等.康贝防治奶牛隐性乳房炎疗效观察及对乳汁体细胞数的影响[J].中国兽医杂志,2002,38(6):11-13.
[1] DUAN Hongbin, ZHAO Xinchun. A Review of Alternative Therapies to Antibiotics for Bovine Mastitis [J]. China Dairy, 2026, 0(5): 63-70.
[2] JI Beibei, BAO Beibei, CAI Kaifeng. Research Progress in Detection Techniques for Mycoplasma bovis [J]. China Dairy, 2026, 0(4): 49-56.
[3] YANG Shengnan, LI Yan, ZHANG Yanqi, LI Guohua, SUN Rongzhao, LIU Xin, DUAN Xiaoxiao. Research on the Therapeutic Effect of Pulsatilla Saponin Injection on Clinical Bovine Mastitis [J]. China Dairy, 2026, 0(1): 58-64.
[4] ZHANG Jianbo, HEI Lixin, PEI Tingfu, ZHU Gaozhao, HU Zhisheng. Bovine staphylococcal mastitis and its Defense Mechanisms [J]. China Dairy, 2025, 0(8): 37-46.
[5] FU Guangda, LIU Weihong, HU Zhisheng. Research Progress on the Relationship Between Staphylococcus aureus Virulence Factors and Antibiotic Resistance in Bovine Mastitis [J]. China Dairy, 2025, 0(7): 90-99.
[6] LIU Weihong, FU Guangda, WANG Guandong, LI Xiaoming. Treatment Approaches and Management Strategies for Bovine Mastitis [J]. China Dairy, 2025, 0(12): 36-42.
[7] LI Xuejun. Investigation on the Efficacy of Compound Chinese Herbs on Pasteurellosis in Dairy Cows in Large-scale Dairy Farms [J]. China Dairy, 2024, 0(6): 64-68.
[8] LIU Minqing, WANG Dening, ZHANG Wenhui, LIU Yixuan, DONG Qing, JIANG Xuefeng, FENG Haipeng, ZHANG Kang. Pathogen Isolation and Identification of Dairy Cow Mastitis in Some Areas of Gansu and Ningxia [J]. China Dairy, 2024, 0(4): 36-41.
[9] ZHANG Taibiao, ZHU Xinrong, ZHANG Caizhen, LU Changbin. Research of Using Internal Teat Sealant Combining Different Drugs Dry-off Prevention of Mastitis in Dairy Cows [J]. China Dairy, 2022, 0(8): 24-28.
[10] WANG Zhenyu, WANG Zeqing, LIU Zhanhong, MAN Jinlong, SUN Shifeng, LI Hongkui, LV Xiaohui, WANG Zhixue. Traditional Chinese Veterinary Medicine for Clinical Mastitis In Dairy Cows [J]. China Dairy, 2022, 0(7): 30-35.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © 2018 Editorial Board of China Dairy
Powered by Beijing Magtech Co. Ltd