China Dairy ›› 2022, Vol. 0 ›› Issue (1): 58-62.doi: 10.12377/1671-4393.22.01.11

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Isolation,Identification and Drug Sensitivity Test of Pathogens Causing Mastitis in 3 Large-scale Dairy Farms in Xinjiang

CHEN Jie1, WEI Yong2, WANG Chenyu1, CAO Mengyuan1, XIE Jingang2, QI Yayin1,*   

  1. 1College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832000;2Xinjiang Tian’ao Animal Husbandry Co.,LTD.,Kuitun Xinjiang 833200;
  • Online:2022-01-25 Published:2022-02-22

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Abstract: Dairy mastitis is one of the most common diseases in dairy cattle,which brings huge economic losses to dairy farming industry. To investigate the incidence and prevalence of mastitis of dairy cows in large-scale dairy farms in Xinjiang,the field epidemiological investigation and laboratory examination,pathogen isolation,identification and drug resistance analysis were carried out on clinical mastitis cattle from 3 large-scale dairy farms in Xinjiang. A total of 243 milk samples with somatic cell count ≥5 million /mL were collected,and 231 suspected gram-negative enterobacter were isolated by continuous streak culture on Mcconkay agar plate and Eosin-Meilan agar plate,combined with smear,staining and microscopic examination. Escherichia coli was identified as the main pathogen causing clinical mastitis of dairy cows in these three large-scale dairy farms. Results：187 strains of Escherichia coli were identified by PCR,and the isolation rate was 77%,drug sensitivity results showed that Escherichia coli was highly sensitive to cefotaxime,streptomycin,gentamicin,amikacin and ciprofloxacin.

Key words: mastitis, Escherichia coli, isolation and identification, drug sensitive test

CLC Number: 

  • S858.23
[1] Ganda E K,Bisinotto R S,Decter D H,et al.Evaluation of an on-farm culture system (Accumast)for fast identification of milk pathogens associated with clinical mastitis in dairy cows[J]. PLoS One,2016,11:e0155314.
[2] 王洪伟. 不同程度奶牛乳房炎乳中相关酶与急性蛋白的研究:[硕士论文][D]. 哈尔滨:东北农业大学,2010.
[3] 徐继英,刘俊林,霍生东,等.致奶牛乳房炎大肠杆菌的分离与鉴定[J]. 中兽医医药杂志,2012(3):20-24.
[4] 王剑锋. 辽宁地区个体奶牛场乳房炎流行情况的调查与综合防治:[硕士论文][D]. 辽宁:沈阳农业大学,2020.
[5] Ashraf A,Imran M,Diagnosis of bovine mastitis:from laboratory to farm[J] .Tropical Animal Health and Production,2018,50:1193-1202.DOI:10.1007/s11250-018-1629-0
[6] 项开合. 丹东地区奶牛隐性乳房炎主要病原菌的分离与鉴定[J]. 黑龙江畜牧兽医,2015(19):187-188.
[7] 蒋增海,王成龙,宋浩,等. 一株致多发性浆膜炎猪源肠外E.coli的分离与鉴定[J]. 现代牧业,2020,4(1):1-5.
[8] Jorgensen H J,Mork T,Rorvik L M.The occurrence of Staphylococcus aureus on a farm with small-scale production of raw milk cheese[J]. Journal of Dairy Science,2005,88(11):3810-3817.
[9] 林向鑫. 奶牛乳房炎的诊断及防治[J]. 兽医导刊,2021(3):115-116.
[10] Lippolis J D,Holman D B,Brunelle B W,et al. Genomic and transcriptomic analysis of escherichia coli strains associated with persistent and transient bovine mastitis and the role of colanic acid[J].Infection and Immunity,2018,86(1):DOI:10.1128/AIA.00566-17.
[11] 廖智慧,陈志军,李晓,等.衡水地区奶牛乳房炎源大肠杆菌分离鉴定、血清型及耐药性分析[J].黑龙江畜牧兽医,2018(2):107-108.
[12] 苑晓萌,赵效南,李璐璐,等.山东地区乳房炎牛奶中大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2021,48(1):312-323.
[13] Heikkila A M,Liski E,Pyorala S,et al.Pathogen-specific production losses in bovine mastitis[J]. Journal of Dairy Science,2018,101(10):9493-9504.
[14] 黄妍梅,王志海,邵芹,等. 奶牛大肠杆菌性乳房炎的诊治及预防[J]. 养殖技术顾问,2008(4):68-68.
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[2] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 79 -81 .
[3] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 6 -9 .
[4] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 57 -61 .
[5] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 50 -51 .
[6] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 42 -43 .
[7] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 38 -39 .
[8] . [J]. Bulletin of Agricultural Science and Technology, 2018, 0(5): 34 -35 .
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