China Dairy ›› 2022, Vol. 0 ›› Issue (9): 29-33.doi: 10.12377/1671-4393.22.09.06

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Effects of Major Royal Jelly Proteins to Cryopreservation Solution on Sperm Performance

LI Ziqing1, HUANG Jinming2, SHEN Lirong3, SHI Fangxiong1,*   

  1. 1 College of Animal Science and Technology,Nanjing Agricultural University,Nanjing Jiangsu 210095;
    2 Shangdong Academy of Agricultural Science Institute of Animal Husbandry and Veterinary Medicine,Jinnan Shandong 250100;
    3 College of Biosystems Engineering and Food Science Zhejiang University,Hangzhou Zhejiang 310058
  • Online:2022-09-25 Published:2022-09-30

Abstract: [Objective] As an indispensable technical link of artificial insemination,semen cryopreservation is of vital significance to the reproduction and preservation of high-quality livestock populations. At present,the quality of cryopreserved semen varies due to factors such as the composition of the cryopreserved semen,the freezing method,and external oxidative stress. Dietary Royal jelly (RJ) has been proven to improve the quality of frozen semen. Major Royal jelly Proteins (MRJPs) as the main dry matter of RJ has extremely high biological activity and antioxidant capacity. [Method] To improve the quality of cryopreserved sperm, different concentrations of the freeze-powder of MRJPs(0 g/25 mL,0.01 g/25 mL,0.02 g/25 mL,0.03 g/25 mL and 0.04 g/25 mL)were added to the bull semen cryopreserved in this study. The spermatozoa were thawed after 48 h of cryopreservation,and related parameters such as total sperm motility, progressive motility,and sperm abnormality were evaluated. [Result] Adding 0.02 g,0.03 g and 0.04 g groups were all significantly decreased the total sperm mobility,fast forward mobility,slow forward mobility,progressive mobility (P< 0.05) in a concentrations-dependent manner. While,there were no significant differences in the sperm abnormality and situ motile sperm rate among groups. [Conclusion] Addition of MRJPs to the semen cryopreservation fluid would inhibit the motility of cryopreserved sperm. What kind ways of MRJPs can improve sperm quality or not still need further researches.

Key words: MRJPs, sperm motility, anti-oxidation, freeze-thaw

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