Abstract: Immobilized microbial system has a wide application prospect in different fields. However,for a long time,the research on the physiological characteristics of immobilized cells lags behind its application,which hinders the practical application of immobilized microorganisms. In order to develop carrier materials with two functions of expanded training and immobilization,taking the number of microorganisms as the detection index,the effects of single factors such as the type,ratio and temperature of the composite carrier on the bacterial absorption of the carrier were investigated. The results showed that the best storage temperature of bacteria ZX5 and ZH9 was 40 ℃,but the optimal composite carrier of ZX5 was C, while the optimal composite carrier of ZH9 was B.The amount of wheat bran and sawdust of composite carrier C is higher than that of composite carrier B,but the content of diatomite is much lower than that of composite carrier B. The permeability and nutrient content of wheat bran and sawdust are much higher than that of diatomite. It can be seen that ZX5 needs more oxygen and nutrients than ZH9.The optimum storage temperature of fungus ZZ9 is 30 ℃,the best composite carrier is A,and the content of wheat bran accounts for 50.0%,indicating that ZZ9 has a high demand for nutrients.

Key words: dry cow dung, composite carrier, adsorbent, microorganism