China Dairy ›› 2024, Vol. 0 ›› Issue (11): 120-132.doi: 10.12377/1671-4393.24.11.20

• QUALITY CONTROL • Previous Articles     Next Articles

Method Validation Study for the Determination of Aflatoxin M1 Content in Milk by High Performance Liquid Chromatography

JI Kunfa, YANG Aijun*, HE Ying, YANG Meifeng, LI Zhifeng, CHEN Jinxuan   

  1. Guangdong Yantang Dairy Co., Ltd., Guangzhou Guangdong 510700
  • Online:2024-11-25 Published:2024-12-10

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Abstract: According to GB 5009.24—2016 National Standard for Food Safety,Determination of Aflatoxins M1 Group in Food,the second method was extracted by methanol–water solution,a method for the determination of aflatoxins M1 in milk by high performance liquid chromatography with high performance liquid chromatography was developed,the detection limit,quantitative limit,linear range,accuracy and repeatability precision were tested. The results showed that the aflatoxins M1 in milk had a good linearity in the range of 0.002 50~2.02 ng/mL,and the correlation coefficient was larger than that of the 0.999. The detection limit of the instrument was 0.003 59 μg/kg,and that of the method was 0.001 80 μg/kg,the quantitative limit of the instrument was 0.010 8 μg/kg,the quantitative limit of the method was 0.005 40 μg/kg,the recoveries were 95.80%~103.37%,the repeatability precision was 0.46%~1.66%,all accord GB 5009.295—2023 The Requirements of National Standard for Food Safety General Rules for Verification of Chemical Analysis Methods.

Key words: high performance liquid chromatography, milk, aflatoxins M1, method validation

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