高效液相色谱法检测液态奶中苯甲酸、山梨酸含量的优化研究

doi:10.12377/1671-4393.25.07.21

China Dairy ›› 2025, Vol. 0 ›› Issue (7): 126-130.doi: 10.12377/1671-4393.25.07.21

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Optimization Study on the Detection of Benzoic Acid and Sorbic Acid in Liquid Milk by High Performance Liquid Chromatography

DU Xiaomeng, CAO Wenyan^*, WANG Meina, ZHAI Jing, HOU Xiaofeng, ZHOU Tianjun, MA Jianjun

Baoding Mengniu Beverage Co.,Ltd.,Hebei Province Key Laboratory of Intelligent Testing Technology for Dairy Products,Baoding Hebei 072450

Online:2025-07-25 Published:2025-08-04

Abstract

Abstract: [Objective] The contents of benzoic acid and sorbic acid in liquid milk were determined by optimizing the liquid chromatography method in the first step of “GB 5009.28-2016 National Food Safety Standard - Determination of Benzoic Acid, Sorbic Acid and Sodium Saccharin in Foods”,and the extraction effect of the optimized method was verified. [Method] The applicability of this optimized method was verified by monitoring the recovery rate detection,precision detection and repeatability detection,and the data was accumulated. [Result] The optimized method had a higher extraction effect,the precision meets the requirements,and the recovery rate was closer to the true value. [Conclusion] The optimized method was more adequate for the extraction of benzoic acid and sorbic acid.It could be used as anauxiliary in the daily detection of complex matrix samples.

Key words: optimized method, extraction effect, recovery rate, standard curve, resolution

DU Xiaomeng, CAO Wenyan, WANG Meina, ZHAI Jing, HOU Xiaofeng, ZHOU Tianjun, MA Jianjun. Optimization Study on the Detection of Benzoic Acid and Sorbic Acid in Liquid Milk by High Performance Liquid Chromatography[J].China Dairy, 2025, 0(7): 126-130.

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Optimization Study on the Detection of Benzoic Acid and Sorbic Acid in Liquid Milk by High Performance Liquid Chromatography

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