China Dairy ›› 2021, Vol. 0 ›› Issue (9): 97-101.doi: 10.12377/1671-4393.21.09.18

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Pathogens Detection and Analysis of Calf Diarrhea in a Large-scale Dairy Farm

RUAN Jixiang1, LIU Wenxiao2, JIANG Bo2, XU Jian2, LI Yongqing2, CHENG Jing2,*   

  1. 1China Animal Husbandry Industry Co.,Ltd.,Beijing 100070;
    2Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097
  • Online:2021-09-25 Published:2021-09-29

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Abstract: In order to investigate and research the main reasons of calf diarrhea in a large-scale dairy farm,blood samples and faeces from sicken calfs were used for pathogen detection of bacteria,viruses and parasites in this study. The results showed that the antibody positive rate of bovine viral diarrhea/mucosal disease virus, bovine rotavirus and bovine coronavirus were 65%, 25.00% and 15.00% respectively. One campylobacter coli and one campylobacter jejuni were isolated. In all stool and blood samples, 4 stool samples and 1 blood sample were positive for bovine coronavirus, 2 stool samples and 1 blood sample were positive for bovine rotavirus, 1 stool sample and 1 blood sample were positive for bovine BVDV and 1 stool sample was both positive for bovine BVDV and coronavirus. The positive rate of cordyceps oocysts was 77.78%,ascaris eggs were found in 1 fecal sample and other angiosperm eggs were found in 2 fecal samples by microscopic examination. It's showed that the causes of massive calf diarrhea in this dairy farm were the decrease of immunity caused by high temperature and humidity in summer, which led to the infection of multiple pathogens.

Key words: calf diarrhea, pathogens, antibody, detection

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