Abstract: This article mainly focused on the the species and mechanism of milk clotting with rennet,homemade lamb rennet and the determination method of enzyme activity,which played an important role on guiding family farms and small goat milk enterprises to make breakthrough on rennet homemade core technology,and also made innovations on goat cheese research and development,filled the blank of the domestic goat cheese market and rich the variety of goat milk products.

Key words: rennet, lamb abomasum, activity determination, goat milk cheese