China Dairy ›› 2024, Vol. 0 ›› Issue (3): 65-70.doi: 10.12377/1671-4393.24.03.12

• QUALITY CONTROL • Previous Articles     Next Articles

Detection Method for Acesulfame Potassium,Benzoic Acid,Sorbic Acid,and Saccharin Sodium in Yogurt

LING Ling, ZHOU Yu, YUAN Yuan, AN Na   

  1. Shijingshan District Center for Disease Control and Prevention,Beijing 100043
  • Online:2024-03-25 Published:2024-04-24

Abstract: [Objective] To establish a method for the simultaneous detection of four food additives, including acesulfame potassium, in yogurt using high-performance liquid chromatography . [Method] Yogurt samples were precipitated with a protein precipitant and then centrifuged at high speed. The supernatant was filtered through a 0.22 µm pore size filter membrane before being measured by the instrument. A C18 chromatographic column was used, with methanol and a 0.02 mol/L ammonium acetate solution (without pH adjustment) as the mobile phase, and the detection wavelength was set at 227 nm. [Result] The linear range of the four food additives was 1.00-50.0 mg/L, with correlation coefficients above 0.9999, and recovery rates between 91.2% and 104.8%. [Conclusion] This method is simple, rapid, and can be used for the simultaneous detection of acesulfame potassium, benzoic acid, sorbic acid, and sodium saccharin in yogurt.

Key words: high-performance liquid chromatography, food additives, simultaneous detection

[1] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T5009.140—2003 饮料中乙酰磺胺酸钾的测定.
[2] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局.GB5009.140—2023 食品安全国家标准食品中乙酰磺胺酸钾的测定.
[3] 中华人民共和国卫生部,中国国家标准化管理委员会.GB5009.28—2016 食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定.
[4] 全国法制计量管理计量技术委员会.JJF1059.1—2012 测量不确定度评定与表示.
[5] 王萍,陈国征,吴佳敏.甲醇沉淀-高效液相色谱法同时测定奶茶中5 种添加剂[J].中国卫生检验杂志,2021,31(1):28-30.
[6] 秦玫,郭志明,聂卫卫.超滤管过滤-超高效液相色谱法测定奶茶、乳饮品中糖精钠、安赛蜜和山梨酸[J].中国卫生检验杂志,2021,31(6):678-679,683.
[1] REN Jingbo, REN Lizhe. Application of Food Additives in Fermented Dairy Products [J]. China Dairy, 2021, 0(4): 85-88.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!