China Dairy ›› 2024, Vol. 0 ›› Issue (10): 64-69.doi: 10.12377/1671-4393.24.10.12

• QUALITY CONTROL • Previous Articles     Next Articles

Application of Flow Cytometry in the Detection of Commercial Sterility of Dairy Products

GUO Jing, WANG Dan, ZHANG Shuai, QI Guoxin, LI Hui, ZHANG Shuli, CHEN Xiaomin   

  1. Testing Laboratory,Mengniu Dairy Shangzhi Co.,Ltd.,Shangzhi Heilongjiang 150600
  • Online:2024-10-25 Published:2024-11-08

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Abstract: [Objective] To clarify the efficiency and accuracy of flow cytometry technology in commercial aseptic testing of dairy products, and to understand the necessity and value of rapid microbial detection technology. [Method] Introduce the definition, principles, and methods of flow cytometry technology, and compare it with the commercial sterility testing methods in the national standard. [Result] Flow cytometry technology and the commercial sterility testing method in the national standard showed commercial sterility for samples without added microorganisms, and non-commercial sterility for samples with added target microorganisms. The test results were consistent. [Conclusion] Flow cytometry technology for detecting commercial sterility and the commercial sterility testing methods in national standards can achieve the same screening purpose in testing, and have advantages in convenience, accuracy, stability, operability, timeliness, etc. It can quickly release products and is of great significance for quality judgment.

Key words: microorganism, flow cytometry, dairy product, rapid detection technology

[1] 苏凤贤,张宝善,曹稳.食品微生物快速检测技术应用进展[J].陕西农业科学,2007(2):78-81.
[2] 闫雪,姚卫蓉,钱和.国内外食品微生物快速检测技术应用进展[J].食品科学,2005,26(6):269-272.
[3] 邹小龙,姜川,郝大伟.食品微生物快速检测技术研究进展[J].食品研究与开发,2012,33(8):226-229.
[4] 中华人民共和国国家卫生和计划生育委员会.GB4789.26—2013食品安全国家标准食品微生物学检验商业无菌检验[S].
[5] 国家市场监督管理总局、国家标准化管理委员会.GB/T 38488—2021微生物快速测定方法[S].
[6] 林木娣,黄思韵,杨爱君,等.Promicol ATP微生物快速检测系统在UHT灭菌奶商业无菌检测上的应用研究[J].中国乳业,2017(6):69-74.
[7] 陶文静. 食品微生物快速检测问答汇总[J].食品安全导刊,2017(3):28-30.
[8] 祁岩,游春苹.流式细胞术在乳品微生物检测中的应用[J].食品与机械,2023,39(2):221-226.
[9] 李萍,温平威,许恒毅,等.流式细胞术在食源致病菌检测中应用的研究进展[J].食品工业科技,2013,34(14):375-379.
[10] 杨莉婷,何丽,何海宁,等.流式细胞术对生乳中微生物检测的应用研究[J].广西师范大学学报(自然科学版),2017,35(2):112-116.
[11] 王宁. 流式细胞术检测牛乳中大肠杆菌数及细菌总数的研究[D].哈尔滨:东北农业大学,2007.
[12] 刘新星,霍转转,云慧,等.流式细胞术在细菌快速检测中的应用[J].微生物学通报,2014,41(1):161-168.
[13] 孔晓雪,韩衍青,付勇,等.流式细胞术在超高压诱导大肠杆菌O157:H7亚致死研究中的应用[J].食品科学,2018,39(3):135-131.
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