China Dairy ›› 2022, Vol. 0 ›› Issue (8): 65-69.doi: 10.12377/1671-4393.22.08.15

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Verification of Determination Method of Cholesterol in Milk Powder

CUI Chengyuan   

  1. The Great Northern Wilderness Wandashan Dairy Co.,Ltd.,Harbin Heilongjiang 150078
  • Online:2022-08-25 Published:2022-09-02

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Abstract: In order to improve the detection efficiency of cholesterol in daily milk powder,a more convenient gas chromatography method was established in this paper.The sample of milk powder was saponified by ethanol and potassium hydroxide solution,and then the cholesterol component in the sample solution was extracted by petroleum ether. Finally,the extract was concentrated to near dryness by a parallel concentrator,then the volume was fixed by n-hexane,and the content was quantified by gas chromatography and external standard method.This method has the advantages of low detection limit,high precision,high accuracy,simple operation process,higher efficiency and simplicity.

Key words: cholesterol, gas chromatography, saponification, concentration, external standard method

CLC Number: 

  • TS207.3
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