China Dairy ›› 2022, Vol. 0 ›› Issue (8): 65-69.doi: 10.12377/1671-4393.22.08.15

Previous Articles     Next Articles

Verification of Determination Method of Cholesterol in Milk Powder

CUI Chengyuan   

  1. The Great Northern Wilderness Wandashan Dairy Co.,Ltd.,Harbin Heilongjiang 150078
  • Online:2022-08-25 Published:2022-09-02

PDF (PC)

113

Abstract: In order to improve the detection efficiency of cholesterol in daily milk powder,a more convenient gas chromatography method was established in this paper.The sample of milk powder was saponified by ethanol and potassium hydroxide solution,and then the cholesterol component in the sample solution was extracted by petroleum ether. Finally,the extract was concentrated to near dryness by a parallel concentrator,then the volume was fixed by n-hexane,and the content was quantified by gas chromatography and external standard method.This method has the advantages of low detection limit,high precision,high accuracy,simple operation process,higher efficiency and simplicity.

Key words: cholesterol, gas chromatography, saponification, concentration, external standard method

CLC Number: 

  • TS207.3
[1] 郭玮,陈方俊,潘柏申.胆固醇检测方法的发展历史及标准化[J].检验医学,2013,28(11):970-974.
[2] 王启辉,刘洋,张经华.生物体内固醇类物质检测方法的研究进展[J].现代科学仪器,2010,2:176-179,183.
[3] 张晓儒. 内蒙古家畜胆固醇含量测定及两种检测方法比较:硕士论文[D].呼和浩特:内蒙古农业大学,2017.
[4] 朱炳祺,刘柱,陈万勤.食品中胆固醇的检测方法的实验室转移确认验证[R].中国药学会第二届药物检测质量管理学术研讨会资料汇编,2015.
[5] 张庆波. 气相色谱质谱法检测食品中胆固醇含量的研究:硕士论文[D].长春:吉林大学,2013.
[6] 徐志红,王金宇,李淑芬.气相色谱法测定鹿茸萃取物中胆固醇的含量[J].食品科学,2007,6(28):269-271.
[7] 龚蕊,苗虹,金绍明.气相色谱-质谱法测定食用油中氯丙醇酯的含量方法验证研究[J].中国食品卫生杂志,2016,3:333-339.
[8] 朱燕萍. 气相色谱法测定食品中乙酰丙酸的方法验证[J].食品界,2018,6:94-98.
[1] HUANG Zhong, LIANG Yiyi. Optimization of Determination of Inositol in Milk and Its Products by Gas Chromatography [J]. China Dairy, 2022, 0(2): 89-93.
[2] WANG Siyu. Application of Evaporation and Concentration Process in Infant Formula Milk Powder Processing [J]. China Dairy, 2022, 0(1): 73-76.
[3] CHEN ZengXin, PAN YunYun, YAN ZeWen, WANG Heng. The Application of Gas Chromatography-Mass Spectrometrography Technology in the Detection of Dairy Products [J]. China Dairy, 2021, 0(3): 69-72.
[4] LU Yujian, Li Yongzhen, Fang Zhiwei, Shi Zhengxiang, Wang Chaoyuan. Research Progress On Particulate Matter Harmful Effects and Its Characteristics from Dairy Barn [J]. China Dairy, 2021, 0(11): 23-33.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © 2018 Editorial Board of China Dairy
Powered by Beijing Magtech Co. Ltd