China Dairy ›› 2022, Vol. 0 ›› Issue (12): 58-62.doi: 10.12377/1671-4393.22.12.11

• DISEASE PREVENTION • Previous Articles     Next Articles

Isolation,Identification and Drug Resistance Analysis of Escherichia coli from Dairy Cow Mastitis in Some Areas of Xinjiang

JIA Limin1, CAI Kuojun1,2,*   

  1. 1Urumqi Animal Disease Control and Diagnosis Center,Urumqi Xinjiang 830063;
    2Xinjiang Agriculture University,Urumqi Xinjiang 830052
  • Online:2022-12-25 Published:2023-01-10

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Abstract: [Objective]In order to understand the prevalence and drug resistance of Escherichia coli in dairy cow mastitis in Xinjiang. [Method]The Escherichia coli from dairy cow mastitis in some areas of Xinjiang were isolated,cultured,stained and identified by fluorescent PCR. The drug sensitivity test of the isolated strains was carried out by disk method. [Result]63 strains of Escherichia coli were isolated from 200 dairy cow mastitis samples,and the isolation rate was 31.5%. The isolates were most resistant to penicillin （93.65%） and most sensitive to meropenem （98.14%）.The isolates were resistant to other antibiotics in different degrees. [Conclusion]The results showed that Escherichia coli of dairy cow mastitis was widespread and had high drug resistance in some areas of Xinjiang.

Key words: dairy cow mastitis, Escherichia coli, isolation and identification, fluorescence PCR, drug resistance

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