China Dairy ›› 2022, Vol. 0 ›› Issue (12): 58-62.doi: 10.12377/1671-4393.22.12.11

• DISEASE PREVENTION • Previous Articles     Next Articles

Isolation,Identification and Drug Resistance Analysis of Escherichia coli from Dairy Cow Mastitis in Some Areas of Xinjiang

JIA Limin1, CAI Kuojun1,2,*   

  1. 1Urumqi Animal Disease Control and Diagnosis Center,Urumqi Xinjiang 830063;
    2Xinjiang Agriculture University,Urumqi Xinjiang 830052
  • Online:2022-12-25 Published:2023-01-10

PDF (PC)

106

Abstract: [Objective]In order to understand the prevalence and drug resistance of Escherichia coli in dairy cow mastitis in Xinjiang. [Method]The Escherichia coli from dairy cow mastitis in some areas of Xinjiang were isolated,cultured,stained and identified by fluorescent PCR. The drug sensitivity test of the isolated strains was carried out by disk method. [Result]63 strains of Escherichia coli were isolated from 200 dairy cow mastitis samples,and the isolation rate was 31.5%. The isolates were most resistant to penicillin （93.65%） and most sensitive to meropenem （98.14%）.The isolates were resistant to other antibiotics in different degrees. [Conclusion]The results showed that Escherichia coli of dairy cow mastitis was widespread and had high drug resistance in some areas of Xinjiang.

Key words: dairy cow mastitis, Escherichia coli, isolation and identification, fluorescence PCR, drug resistance

[1] Igbal M R.Bovine mastitis in Fiji:economic implications and management—a review[J]. Canadian Center of Science and Education,2021(10):162-174.
[2] 买尔哈巴·吾斯曼,左佳坤,吕朝阳,等.2020年国内部分地区奶牛乳腺炎源大肠杆菌的分子流行病学调查[J/OL].中国动物传染病学报:1-12[2022-09-02].
[3] 于伟伟,乔军,孟庆玲,等. 奶牛乳房炎大肠杆菌新疆分离株系统分群及其耐药特性与毒力因子分布研究[J]. 中国奶牛,2017(10):30-35.
[4] 刘晓芬,陈法杰. 新疆乳制品加工业发展现状及对策[J]. 食品工业,2020, 41(8):262-265.
[5] 陈杰,魏勇,王晨豫,等. 新疆部分地区9个奶牛场致奶牛乳房炎主要病原菌的检测与药敏试验[J]. 中国牛业科学,2021,47(6):9-15.
[6] 常军帅,张琪,林津如,等. 新疆部分地区临床型乳房炎源大肠杆菌的调查分析[J]. 中国奶牛,2022(1):21-26.
[7] 刘肖利,刘璐瑶,李镔罡,等. 奶牛乳房炎源大肠埃希氏菌的耐药性分析和毒力基因检测[J].动物医学进展,2022,43(1):46-51.
[8] 张鹏飞,王婷,钟楠,等. 奶源大肠杆菌的分离鉴定及耐药性分析[J]. 食品安全质量检测学报,2020,11(9):2896-2903.
[1] OU Xiuling, CAI Kuojun, XU Min. Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus from Raw Camel Milk [J]. China Dairy, 2022, 0(8): 29-33.
[2] ZHAI Hongrui, ZHAN Dongli, GUO Qing. Study on in Vitro Antibacterial Effect of 7 Chinese Herbs on Pathogenic Bacteria of Dairy Cow Nastitis [J]. China Dairy, 2022, 0(8): 34-37.
[3] ZHANG Liqiang, LEI Jing. Isolation,Identification and Drug Sensitivity Test of Staphylococcus aureus from Large-scale Pasture in Ningxia [J]. China Dairy, 2022, 0(3): 46-49.
[4] ZHANG Huan, HU Chaohui, GOU Yaopeng, WANG Liang, QI Yayin. Isolation,Identification,Virulence and Drug Resistance Analysis of Four Strains of Pathogenic Escherichia coli Causing Diarrhea of Calves [J]. China Dairy, 2022, 0(12): 77-81.
[5] HU Shaozhen, LI Lianrui. Isolation and Identification of Pseudomonas From Spoiled Raw Milk and Determination of Protease Activity [J]. China Dairy, 2022, 0(10): 77-81.
[6] CHEN Jie, WEI Yong, WANG Chenyu, CAO Mengyuan, XIE Jingang, QI Yayin. Isolation,Identification and Drug Sensitivity Test of Pathogens Causing Mastitis in 3 Large-scale Dairy Farms in Xinjiang [J]. China Dairy, 2022, 0(1): 58-62.
[7] CHEN Mingjie, CAO Mengyuan, WANG Chenyu, YAN Xueqi, QI Yayin. Isolation, Identification and Drug Sensitivity Test of Mastitis Milk-like Bacteria in a Pasture in Xinjiang [J]. China Dairy, 2021, 0(7): 59-62.
[8] WANG Chun'ao. Diagnosis and Control of Calf Diarrhea in Scaled farms [J]. China Dairy, 2021, 0(10): 2-8.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © 2018 Editorial Board of China Dairy
Powered by Beijing Magtech Co. Ltd