China Dairy ›› 2023, Vol. 0 ›› Issue (12): 41-46.doi: 10.12377/1671-4393.23.12.09

• DISEASE PREVENTION • Previous Articles     Next Articles

Analysis of Drug Resistance and Virulence Gene Detection of Streptococcus uberis in Dairy Cow with Endometritis

LIU Zhiqiang   

  1. Xianghe County Agriculture and Rural Bureau,Langfang Hebei 065400
  • Online:2023-12-25 Published:2024-01-12

Abstract: [Objective]To understand the drug resistance and virulence genes of Streptococcus uberis in dairy cows with endometritis.[Method]76 uterine mucus samples of streptococcus uberis in dairy cows with endometritis were collected from 5 dairy farms in Xianghe County.Streptococcus uberis was identified by PCR method.The minimum inhibitory concentration(MIC) of 8 antibiotics against Streptococcus uberis was determined by micro-broth dilution method to determine its drug resistance.PCR method was used to detect Streptococcus uberis carrying 10 virulence genes.[Result]18 strains of Streptococcus uberis were detected by PCR method,and the total isolation rate was 23.68%.18 strains of Streptococcus lactis were highly resistant to clindamycin, gentamicin and tetracycline, and the resistance rates were 66.67%, 88.89% and 72.22% respectively,respectively.All strains carried slp,sua,gapC,fpb,cfu and pauA,while the detection rates of hasA,hasB and acdA were 38.89%,50.00% and 33.33%,respectively,and hasC was not detected.[Conclusion]Streptococcus uberis was one of the main pathogenic bacteria in dairy cow with endometritis in this area.The drug resistance was serious,and the virulence genes were widely prevalent.The monitoring should be strengthened and the drug should be used rationally.

Key words: dairy cow, endometritis, Streptococcus uberis, drug resistance, virulence gene

[1] 蒋会会,王自力,王晓谦,等. 奶牛子宫内膜炎的免疫防御机能变化及中药防治研究进展[J]. 中国兽医杂志,2015,51(12):67-70.
[2] 许腊梅,冯万宇,王岩,等. 奶牛子宫内膜炎研究进展[J]. 动物医学进展,2008(8):71-74,96.
[3] 谢昆,蒋成砚,王会英,等. 奶牛隐性乳房炎链球菌的分离鉴定及药敏试验[J]. 上海农业学报,2015,31(5):37-41.
[4] Reinoso E B.Bovine mastitis caused by Streptococcus uberis:Virulence factors and biofilm[J]. Microbial & Biochemical Technology,2017,9(5):1-7.
[5] Collado R,Prenafeta A,González-González L,et al.Probing vaccine antigens against bovine mastitis caused by Streptococcus uberis[J]. Vaccine,2016,34(33):3848-3854.
[6] 杨洁,蒋威,沈文祥,等. 奶牛源乳房链球菌毒力与耐药性分析[J]. 动物医学进展,2022,43(1):12-18.
[7] Field T R,Ward P N,Pedersen L H,et al.The hyaluronic acid capsule of Streptococcus uberis is not require for the development of infection and clinical mastitis[J]. Infect Immun,2003,71(1):132-139.
[8] Reinoso E B,Lasagno M C,Dieser S A,et al.Distribution of virulence-associated genes in Streptococcus uberis isolated from bovine mastitis[J]. FEMS Microbiology Letters,2011,318(2):183-188.
[9] Kerro Dego O,Almeida R A,Saxton A M,et al.Bovine intramammary infection associated immunogenic surface proteins of Streptococcus uberis[J]. Microbial Pathogenesis,2018,115:304-311.
[10] Edyta K,Joanna M,Roman W,et al.Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland[J]. BMC Veterinary Research,2017,13(1):398.
[11] Fessia S A,Dieser A S,Raspanti G C,et al.Genotyping and study of adherence-related genes of Streptococcus uberis isolates from bovine mastitis[J]. Microbial Pathogenesis,2019,130,295-301.
[12] 王红均,程邓芳,先宏,等. 17味中草药及其复方对鸽沙门氏杆菌的体外抑菌试验[J].黑龙江畜牧兽医,2019(15):135-137,141.
[13] 王孝武,王旭荣,杨志强,等. 甘肃省某牛场奶牛子宫内膜炎病原菌的分离鉴定及其耐药性分析[J]. 西北农业学报,2014,23(6):16-21.
[14] 凌丁,张业怀,莫文湛,等. 南宁市郊奶牛子宫内膜炎病原菌分离鉴定与耐药性分析[J]. 广东农业科学,2013,40(5):111-115.
[15] 李蕊,马玉忠,李睿文,等. 保定市奶牛子宫内膜炎细菌分离鉴定和药敏试验[J]. 河北农业大学学报,2020,43(5):85-89.
[16] Leigh J A,Lincoln R A.Streptococcus uberis acquires plasmin activity following growth in the presence of bovine plasminogen through the action of its specific plasminogen activator[J]. FEMS Microbiology Letters,1997,154(1):123-129.
[17] Almeida R A,Oliver S P.Antiphagocytic effect of the capsule of Streptococcus uberis[J]. Zentralbl Veterinarmed B,1993,40(9-10):707-714.
[18] Ling E,Feldman G,Portnoi M,et al.Glycolytic enzymes associated with the cell surface of Streptococcus pneumoniae are antigenic in humans and elicit protective immune responses in the mouse[J]. Clinical & Experimental Immunology,2004,138(2):290-298.
[19] Maeda K,Nagata H,Kuboniwa M,et al.Characterization of binding of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase to Porphyromonas gingivalis major fimbriae[J]. Infection and Immunity,2004,72(9):5475-5477.
[20] Zhang H,Yang F,Li X P,et al.Detection of antimicrobial resistance and virulence-related genes in Streptococcus uberis and Streptococcus parauberis isolated from clinical bovine mastitis cases in northwestern China[J]. Journal of Integrative Agriculture,2020,19(11):2784-2791.
[21] 赵祎云,隋国燕,唐峰,等. 兰州地区奶牛临床型乳房炎主要病原菌分离鉴定及耐药性分析[J]. 中国兽医杂志,2020,56(3):91-94.
[22] El Garch F,Youala M,Simjee S,et al.Antimicrobial susceptibility of nine udder pathogens recovered from bovine clinical mastitis milk in Europe 2015—2016:VetPath results[J]. Veterinary Microbiology,2020,245:108644.
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