China Dairy ›› 2024, Vol. 0 ›› Issue (1): 69-73.doi: 10.12377/1671-4393.24.01.13

• QUALITY CONTROL • Previous Articles     Next Articles

Combined Validation of Aflatoxin M1 Detection by Enzyme-linked Immunoassay Techniques and Colloidal Gold Techniques

QIAO Kuan, WANG Luyang, WANG Yanfei, ZHANG Yunxian, SHI Changxu   

  1. Mengniu Dairy（Jiaozuo） Co.,Ltd.,Jiaozuo Henan 454100
  • Online:2024-01-25 Published:2024-02-29

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Abstract: [Objective]To accurately complete the detection of aflatoxin M1 in raw milk.[Method]This paper used a microplate reader and a colloidal gold reader to detect the treatment process and detection data of aflatoxin M1 in raw milk for comparative analysis.[Result]We discovered the advantages and disadvantages of two methods for verifying the operation process,and the test results could meet different needs. The microplate reader could be used for semi-quantitative detection,and the microplate reader could be used for accurate detection and semi-quantitative review. The colloidal gold reader was suitable for rapid qualitative screening.[Conclusion]The two methods could be used together,complement each other,flexibly use the advantages of various equipments,and could efficiently complete various testing requirements.

Key words: colloidal gold reader, microplate reader, aflatoxin M1, raw milk

[1] 农业大词典编辑委员会. 农业大词典[M]. 北京:中国农业出版社,1998.
[2] 朱聪英,应永飞,韦敏珏,等. 液相色谱-串联质谱法测定饲料中黄曲霉毒素的研究[J]. 质谱学报,2010,31(4):240-246.
[3] 张宗城. 乳与乳制品中黄曲霉毒素M1的限量确定及测定方法[J]. 农产品质量与安全,2010(6):36-39.
[4] 林维宣,李继业,田苗. 单克隆免疫亲和柱-高效液相色谱法测定牛乳中黄曲霉毒素M1[J]. 中国乳品工业,2000,28(6):24-26.
[5] 国家质量监督检验检疫总局,中国国家标准化管理委员会. 实验室质量控制规范食品理化检测:GB/T 27404—2008[S]. 北京:中国标准出版社,2008.
[6] 王雄,王艳斐,果旗,等. 黄曲霉毒素直接竞争法ELISA试剂盒的研制及其在花生和花生制品中的应用[J]. 中国饲料,2010(16):37-39.
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