胶体金免疫层析试纸条同时检测生乳中吡虫啉、吡唑醚菌酯、氟虫腈残留的研究

doi:10.12377/1671-4393.22.10.12

Abstract

Abstract: [Objective] To develop a colloidal gold immunochromatography method for simultaneous detection of Imidacloprid，Pyrazole ether ester and Fluorine worm nitrile residues in raw milk. [Method] The colloidal gold solution was prepared by trisodium citrate reduction method, and the colloidal gold standard antibody lyophilized powder was prepared by lyophilization method. The powder was placed in a microporous cup，and the reaction solution was absorbed on the sample pad of colloidal gold test strip after resolution. [Result] The sensitivity of three pesticides，imidacloprid，pyrazoline and fipronil，were 0.1 mg/kg, 0.03mg/kg and 0.02 mg/kg，respectively. The shelf life was 1 year. In accordance with GB2763-2021 National Food Safety Standard Maximum Residue Limits for Pesticides in Food，the maximum residue limits of imidacloprid，pyrazolylosteril and fipronil in raw milk are specified to be 0.1mg/kg，0.03mg/kg and 0.02mg/kg respectively. [Conclusion] This method is simple, rapid，sensitive，stable and accurate，and can be used as a rapid screening method for early detection of agricultural residues.

Key words: fresh milk, imidacloprid, pyrazole ether ester, fluorine worm nitrile, colloidal gold

CHEN Farong, JIN Hongwei, HAN Yanxu, WU Yuehao, QI Yu. Determination of Pesticide Residues in Raw Milk by Colloidal Gold Chromatography[J].China Dairy, 2022, 0(10): 65-71.

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