为了解新疆地区部分规模奶牛场牛病毒性腹泻病（BVD）的流行情况,优化防控措施,以达到建设防控净化场的目的,自2020年11月到2021年7月累计采集新疆5 个地区16 个奶牛场共计26 997 份血清、3 843 份犊牛耳组织进行全群普检。通过使用IDEXX公司牛病毒性腹泻病毒（BVDV）抗原检测试剂盒检测及RT-PCR复检结合测序等方法,淘汰阳性牛,同源性分析流行毒株情况。BVDV血清抗原检测结果为：沙湾某奶牛场BVD阳性率为1.63%（38/2 326）;乌鲁木齐某奶牛场BVD阳性率为0.35%（4/1 132）;其余奶牛场均为阴性。犊牛耳组织抗原检测结果为：乌鲁木齐某牛场BVDV阳性率为1.17%（4/342）;其余奶牛场均为阴性。研究结果揭示,奶牛场通过淘汰BVDV阳性牛,调整免疫程序、制定消毒程序等方法,可有效净化BVD。
In order to understand the prevalence of bovine viral diarrhea disease (BVD) in some large-scale dairy farms in Xinjiang, and to optimize the prevention and control measures for the purpose of building prevention and control and purification farms, a total of 26 997 serum samples and 3 843 calf ears tissues were collected from 16 dairy farms in 5 regions of Xinjiang from November 2020 to July 2021. By using IDEXX BVDV antigen detection kit and RT-PCR retesting combined with sequencing and other methods, positive cattle were eliminated, and homology analysis of the epidemic virus strains. The positive rate of BVDV was 1.63% (38/2 326) in a dairy farm in Shawan. The positive rate of BVD in a dairy farm in Urumqi was 0.35% (4/1 132). All other dairy farms were negative. The positive rate of BVDV was 1.17% (4/342) in a cow farm in Urumqi. All other dairy farms were negative. The results showed that elimination of BVDV positive cattle, adjustment of immunization program and disinfection program could effectively purify bovine viral diarrhea.
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