为调查宁夏回族自治区（简称“宁夏”）银川市规模牧场犊牛病毒性腹泻病毒（BVDV）感染情况,本试验采集某牧场6月龄内犊牛血液和粪便拭子各69 份,采用商品化BVDV抗原检测试剂盒结合RT-PCR检测及微流控芯片检测技术等进行检测。结果表明,商品化BVDV抗原检测、RT-PCR核酸检测方法及微流控芯片检测结果一致,阳性率均为7.24%。结果表明,该牛场BVDV感染率较高,为了做好该病的净化,应加强全群牛只的检测,以防止该病在此牧场进一步蔓延。
In order to investigate the infection of Bovine Viral Diarrhea Virus （BVDV） in calves on a large scale farm in Yinchuan,Ningxia Hui Autonomous Region,69 blood and faecal swabs were collected from calves within 6 months of age on a farm and tested using a commercial BVDV antigen test kit combined with RT-PCR and microfluidic microarray techniques. The results showed that the commercial BVDV antigen test,the RT-PCR nucleic acid test and the microfluidic microarray test were consistent,with a positive rate of 7.24%. The results indicate that the rate of BVDV infection is high in this cattle farm and that in order to do a good job of decontaminating the disease,testing of the whole herd should be strengthened to prevent further spread of the disease in this farm.
[1] Antos A,Miroslaw P,Rola J,et al.Vaccination failure in eradication and control programs for bovine viral diarrhea infection[J]. Frontiers in Veterinary Science,2021,8:688911.
[2] Yesilbag K,Toker E B,Ates O.Ivermectin also inhibits the replication of bovine respiratory viruses(BRSV,BPIV-3,BoHV-1,BCoV and BVDV)in vitro[J] .Virus Research,2021,297: 198384.
[3] Olafson P,Maccallum A D,Fox F H .An apparently new transmissible disease of cattle[J]. The Cornell veterinarian,1946,36(3):205-213.
[4] 李佑民,刘振润.吉林省某奶牛场暴发牛病毒性腹泻——粘膜病及其病毒分离的初步研究[J].兽医大学学报,1981(3):62-64,70.
[5] 赵阳,傅小平.牛病毒性腹泻病毒持续感染对后备奶牛的负作用[J]. 中国乳业, 2010(1):72-73.
[6] 王标. 新疆某规模化牧场犊牛IBR、BVD的诊断与防制:[硕士论文][D].石河子大学,2020.
[7] 张国华,周玉龙,刘琳珊,等. Cp型BVDV在动物体内的分布和其对宿主细胞凋亡的影响[C]// 中国畜牧兽医学会兽医病理学分会学术研讨会暨中国病理生理学会动物病理生理专业委员会学术研讨会. 中国畜牧兽医学会兽医病理学分会;中国病理生理学会动物病理生理专业委员会;宁夏医科大学;宁夏大学, 2014.
[8] 张信军,羊扬,林航,等. 江苏规模化奶牛场BVD与IBR流行病学调查[J]. 中国兽医学报,2018,38(1):69-76.
