磺胺类药物是一类用药简便、价格低廉、性质稳定、抗菌谱广的人工合成抗菌药,但长期或过量使用会出现耐药性和毒副作用,其可通过食物链途径进入人体肠道并影响人类免疫功能。高效液相色谱法是一种集合灵敏度、精密度和准确度的高效筛查方法,磺胺类药物残留检测有利于提高牛奶产品的安全性。本文介绍了磺胺类药物作用机理、耐药性问题、不良反应和检测意义,并总结了牛奶中磺胺类药物最高残留限量规定和检测应用,分析了高效液相色谱法检测优化环节,为高效液相色谱法在牛奶中磺胺类药物残留检测应用提供参考。
Sulfonamides are a kind of synthetic antibiotics with simple use,low price,stable properties and wide antibacterial spectrum. However,long-term or excessive use will lead to drug resistance,toxic and side effects. They can enter the human intestine through the food chain and affect human immune function. High performance liquid chromatography is an efficient screening method with sensitivity,precision and accuracy. The detection of sulfonamides residues is helpful to improve the safety of milk products. This paper firstly introduced the mechanism of action,drug resistance,adverse reactions and detection significance of sulfonamides,and summarized the maximum residue limits and detection applications of sulfonamides in milk,provided reference for the application of high performance liquid chromatography in the detection of sulfonamide drug residues in milk.
[1] 韩博,石玉祥,高健,等.细菌性奶牛乳房炎发生、流行现状及防控研究进展[J].北方牧业,2022(5):28-29.
[2] Gao J,Barkema H W,Zhang L,et al.Incidence of clinical mastitis and distribution of pathogens on large Chinese dairy farms[J]. Journal of Dairy Science,2017,100(6):4797-4806.
[3] 于长泳. 锦州市某奶牛场隐性乳房炎金黄色葡萄球菌耐药分子机制研究[J].黑龙江畜牧兽医,2018(12):124-126.
[4] 杜连启,朱凤妹,李楠.胶体金免疫层析法检测猪肉中3种磺胺类物质残留[J].中国食品学报,2014,14(3):151-160.
[5] 杨华生. 酶联免疫法测定畜产品及牛奶中磺胺类药物残留[J].今日畜牧兽医,2019,35(5):5-7.
[6] 苏明明,代弟,王晓薇,等.放射免疫法快速检测牛血清中磺胺类药物[J].食品安全质量检测学报,2016,7(2):634-638.
[7] 郝晓丽,谷学新,叶能胜,等.毛细管电泳法检测肉类中5种磺胺类药物残留研究[J].药物分析杂志,2009,29(4):579-581.
[8] Cannavan A,Hewitt S A,Blanchflower W J, et al.Gas chromatographic-mass spectrometric determination of sulfamethazine in animal tissues using a methyl/trimethylsilylderivativel[J]. Analyst,1996,121(10):1457-1461.
[9] 蒋翠红. 高效液相色谱检测水产品磺胺类抗生素的探讨[J].食品安全导刊,2016(11):69.
[10] 张筱艳,方素栎,魏晋梅.牛肉中磺胺类药物残留的检测分析[J].中国草食动物科学,2016,36(3):34-37.
[11] 于淼. 养殖业中磺胺类药物残留的危害及现状[J].现代畜牧科技,2015(2):133.
[12] 贾涛. 超高效液相色谱法检测饲料中氟喹诺酮类药物的方法研究[J].饲料与畜牧,2015(1):50-54.
[13] 刘志科,张秋雨,陈创夫.一例犊牛磺胺药中毒症状的观察与分析[J].广东畜牧兽医科技,2017,42(5):13-16.
[14] 白天红,胡淑敏.高效液相色谱法测定奶粉中磺胺类药的物残留量的研究[J].中国乳业,2009(4):43-44.
[15] Ko E,Song H,Park J H.Direct competitive enzyme-linked immunosorbent assay for sulfamethazine.[J]. Journal of Veterinary Medical Science, 2000,62(10):1121-1123.
[16] 于淼. 养殖业中磺胺类药物残留的危害及现状[J].现代畜牧科技,2015(2):133.
[17] 章一. 高效液相色谱法的优点与实际问题中的应用[J].化工管理,2014(24):127.
[18] 王伟,张鹏飞,王丹慧,等.高效液相色谱法测定牛奶中十二种磺胺类药物残留[J].中国乳品工业,2015,43(5):54-56.
[19] 赵寅,卢玉,刘桂亮,等.固相萃取-高效液相色谱法同时测定牛奶中22种磺胺类兽药残留[J].分析试验室,2022,41(2):187-191.
[20] 孔庆德. 高效液相色谱法检测牛乳中的磺胺类药残留量[J].畜牧兽医杂志,2013,32(3):4-6.
[21] 曹雪琴,陈俊宇,张永权,等.高效液相色谱法测定牛奶中磺胺类药物残留[J].广州化工,2015,43(21):122-123,166.
[22] 王露,汪怡,杭学宇,等.磁性固相萃取-高效液相色谱法测定牛奶中4种磺胺类药物[J].理化检验(化学分册),2017,53(5):562-565.
[23] 邵金良,杨东顺,王丽,等.固相萃取-高效液相色谱法同时测定牛奶中13种磺胺残留[J].分析试验室,2014,33(11):1322-1325.
[24] 段婕,李兴华,刘坤,等.高效液相色谱-化学发光法检测牛奶中磺胺类药物残留[J].分析化学,2017,45(7):1012-1018.
