Abstract: Grapevine wound fluid is a colorless,odorless,transparent liquid that naturally flows out of the grape branches.Lactic acid bacteria are suitable in grapevine wound fluid. In this project,the grape branches are chopped and soaked,and the lactic acid bacteria culture medium is prepared by adding the nutrient of the lactic acid bacteria to the soaking liquid.The acidity of lactic acid bacteria in the soaking solution was measured by pH meter, and the change of absorbance at 610nm was measured by UV-Vis spectrophotometer.The growth of lactic acid bacteria in grape branch water infusion was studied.The results showed that the acidity of the medium increased gradually with the passage of time, and the corresponding absorbance also increased,and the highest absorbance reached 1.164A^。. The number of lactic acid bacteria reached 1.46 × 10⁶,and the stable period reached six months.The experimental results show that the grape vinegar soaking liquid can be used to prepare a highly stable and pure natural lactic acid bacteria active beverage.

Key words: Lactic acid bacteria, wound fluid, grape branch water infusion, culture medium, suitable