China Dairy ›› 2024, Vol. 0 ›› Issue (1): 45-50.doi: 10.12377/1671-4393.24.01.09

• DISEASE PREVENTION • Previous Articles     Next Articles

Isolation, Identification and Prevention of Main Pathogenic Bacteria of Dairy Goat Mastitis

BAI Qingling1, WANG Bin2   

  1. 1Animal Disease Control and Prevention Center of Yishui County, Linyi Shandong 276400;
    2Daotuo Animal Husbandry and Veterinary Workstation of Yishui County, Linyi Shandong 276400
  • Online:2024-01-25 Published:2024-02-29

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Abstract: [Objective] To isolate and identify the main bacteria causing mastitis in a dairy goat farm in Linyi area of Shandong Province, and to explore its drug sensitivity. [Methods] In this experiment, milk samples of dairy goats with mastitis were collected from a dairy goat farm in Linyi area, Shandong Province, and pathogens were isolated by bacterial culture medium. PCR amplification technology was used for sequencing and database comparison to identify bacteria. The resistance of Escherichia coli and Staphylococcus aureus to antibiotics commonly used in clinical practice was determined by Kirby-Bauer disk diffusion method. [Result] A total of 109 pathogenic bacteria were isolated and identified, among which Escherichia coli and Staphylococcus aureus accounted for the most, 41.28% and 28.44%, respectively. The results showed that Escherichia coli and Staphylococcus aureus had high resistance to penicillin G and clindamycin, but were more sensitive to quinolones and cephalosporins. Among them, Staphylococcus aureus had the highest sensitivity to cefotaxime sodium (97.0%), while Escherichia coli had the highest sensitivity to ofloxacin (73.8%). It is suggested that these two antibiotic drugs can be used to treat mastitis of dairy goats in this farm. [Conclusion] The main bacterial pathogens of mastitis in dairy goats in this farm are Staphylococcus aureus and Escherichia coli, which are the most sensitive to cefotaxime sodium and ofloxacin respectively, and are recommended for clinical treatment.

Key words: dairy goat, mastitis, pathogenic bacteria, isolation and identification, prevention and control measures

[1] 张自由,吕小琳,高继伟,等. 奶山羊繁殖调控技术研究进展[J]. 中国奶牛,2019(12):38-41.
[2] 段振华,黄宇,甘一波,等. 玉林市奶山羊隐性乳房炎调查及病原菌鉴定与耐药分析[J]. 广西畜牧兽医,2017,33(3):140-142.
[3] 郑风英. 滨州市奶山羊临床型乳房炎病原菌的分离鉴定与耐药性分析[J]. 动物医学进展,2014(8):122-125.
[4] 侯伟杰,张立强,高洋,等. 奶山羊乳房炎灭活疫苗的制备及免疫效果检测[J]. 动物医学进展,2014,35(12):18-22.
[5] 孙衍立. 奶山羊乳房炎常用治疗方法[J]. 兽医导刊,2021(19):128-129.
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