China Dairy ›› 2025, Vol. 0 ›› Issue (12): 77-84.doi: 10.12377/1671-4393.25.12.12

• QUALITY CONTROL • Previous Articles     Next Articles

Validation of Folic Acid Detection Methods in Infant Formula and Analysis of Key Control Points

JIN Wei1, ZHANG Huiping1,*, CHEN Chen1, LU Guangye1, TIAN Haoran1, LU Guanghui2, WU Teng2, LIU Junjun1, LI Hao3   

  1. 1 Inner Mongolia Mengniu Dairy Industrial Co.,Ltd.,Hohhot Inner Mongolia 011517;
    2 Inner Mongolia Tekangrui Nutrition Food Co., Ltd., Hohhot Inner Mongolia 011517;
    3 Inner Mongolia Oushimengniu Dairy Products Co., Ltd., Hohhot Inner Mongolia 011517
  • Online:2025-12-25 Published:2026-01-14

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Abstract: [Objective] The detection of folic acid in infant formula is of critical significance for ensuring the healthy growth of infants and young children and for product quality control. Regular participation in detection proficiency testing activities helps enhance the capability level of personnel and provides a guarantee for the improvement of the laboratory's detection ability for folic acid content in infant formula.[Method] This study participated in the proficiency testing project for folic acid detection in infant formula with item number MA-IP-025-042-24-E9326 of the Chinese Academy of Quality and Inspection&Testing. The detection was completed strictly in accordance with the operation instructions provided by the organizer and the detection methods of this laboratory. Based on the specificity and high sensitivity of Lactobacillus rhamnosus to folic acid,in this study,a simple and highly accurate quantitative detection system for folic acid was constructed by using the IfpVitaFast Vitamin B9 Folic Acid test kit in combination with microbiological methods. The folic acid content in the verification sample MA-IP-025-042-24-E9326 was quantitatively detected,and the test results were analyzed and evaluated.[Result] The detected result of folic acid content in sample MA-IP-025-042-24-E9326 was 119.64 μg/100 g,with a Z-value of 0.2,and |Z|≤2. The result was satisfactory.[Conclusion] The test kit method provided accurate detection results,was simple,rapid and efficient to operate,and offers reliable technical support for the analysis of laboratory detection capability of folic acid content in infant formula.

Key words: folic acid, infant formula, microplate reagent kit, validation sample

[1] 吴思敏,严家俊,黄钟标.微生物法测定婴幼儿配方乳粉中叶酸含量不确定度评估[J].中国乳业,2021(7):91-97.
[2] 王晶,芦云,师佳,等. 2 种国标方法检测婴幼儿奶粉中叶酸和泛酸含量比较[J].检验检疫学刊,2019,29(3):77-78,85.
[3] 张明明,黄志深,蒋佳希,等.婴幼儿乳粉叶酸含量两种测定方法的研究[J].轻工科技,2019,35(7):14-16.
[4] 王卫平,郭志平,程五凤,等.维生素,知多少[J].科学世界,2007(7):12-45.
[5] 中国医药教育协会临床合理用药专业委员会,中国医疗保健国际交流促进会高血压分会,中国妇幼保健协会围产营养与代谢专业委员会,等.中国临床合理补充叶酸多学科专家共识[J].医药导报,2021,40(1):1-19.
[6] 毛宏梅,石丽丽,霍军生.个性化叶酸补充预防神经管缺陷[J].卫生研究,2023,52(4):676-681.
[7] 王姝,李泉波,冯晓玲.叶酸补充过量与生殖风险相关性的研究进展[J].中国计划生育学杂志,2023,31(4):982-987.
[8] 陆其聪,陈冬梅,胡春红,等.微生物法检测婴儿配方乳粉中叶酸含量的影响因素[J].食品科技,2021,46(7):273-277.
[9] 许天月,张俊杰,魏家乐,等.叶酸形式、稳定性及天然化叶酸的研究进展[J].食品安全质量检测学报,2024,15(2):266-274.
[10] 围受孕期增补叶酸预防神经管缺陷指南工作组.围受孕期增补叶酸预防神经管缺陷指南(2017)》[J].中国生育健康杂志,2017,28(5):401-410.
[11] 刘佳,陈峰,王丽,.微生物检测叶酸的自动化改良及应用现状[J].分析测试学报,2022,41(8):1156-1162.
[12] 李刚,王艳,张涛.叶酸在饲料行业中的应用现状与发展前景[J].饲料工业,2024,45(8):12-18.
[13] 中华人民共和国卫生部.GB 10765—2021 食品安全国家标准婴儿配方食品[S].
[14] 李璐,马宁.多种方法检测乳粉中菌落总数能力验证样品[J].现代食品,2022,28(2):175-177.
[15] 阴甜甜,张璐,张莹莹,等.婴幼儿配方乳粉中生物素的测定能力验证结果分析[J].食品工业,2023,44(8):173-176.
[16] 韩奕奕,王霞,马颖清,等.乳粉中烟酸和烟酰胺含量测定能力验证结果与分析[J].食品安全质量检测学报,2020,11(17):5879-5885.
[17] 汪腊云,裴华,朱晓薇,等.食品中霉菌和酵母菌计数的检测能力验证结果分析[J].食品安全质量检测学报,2021,12(3):1021-1025.
[18] 中国合格评定国家认可委员会.CNAS—RL02:2018 能力验证规则[S].
[19] 中国团体标准.T/CIFST015—2023 食品中叶酸的测定预包被微孔板式微生物法[S].
[20] 王文娟,陈文青,章明,等.婴幼儿乳粉中叶酸能力验证结果分析[J].江西化工,2024,40(4):11-14,25.
[21] 金政权,屈伟,柴子寅,等.实验室能力验证信息的利用与持续改进[J].石化技术,2025,32(1):325-327.
[22] 王在彬. 如何通过能力验证提高实验室检测能力[J].计量与测试技术,2024,50(11):127-130.
[23] 李学敏. 叶酸的合成及稳定性研究[D].石家庄:河北科技大学,2015.
[24] 魏华,徐锋,徐玉琴.鼠李糖乳杆菌的生物学特性及发酵性能研究[J].食品与发酵工业,2001(9):11-15.
[25] 张晓金,杨丹妮,邹雨虹,等.微生物法测定婴幼儿乳粉中叶酸含量方法优化[J].现代食品,2021,27(13):201-204.
[26] 田浩,王志伟,顾文佳,等.微生物法测定食品中叶酸、泛酸、生物素、维生素B12注意事项和实践[J].中国标准化,2018(17):127-130.
[27] 张艳霞,萧慧珍,于晓君,等.企业微生物实验室能力验证的质量控制[J].生物化工,2021,7(2):120-123,129.
[28] 高娟,高霞,亢玉红.化妆品中微生物检验能力验证结果分析与质量控制探讨[J].药物生物技术,2022,29(4):377-380.
[29] 邵悦,刘永泽,卢福荣,等.2022—2024年食品微生物能力验证的结果分析探讨[J].食品安全导刊,2025(7):58-60,64.
[30] 唐璐子,张洋,周方,等.食品中叶酸不同检测技术研究进展[J].粮食与食品工业,2025,32(1):62-67.
[31] 谢虹. 调饮类食品中添加剂的快速检测技术[J].现代食品,2025(2):219-221.
[32] 苏妙仪,严家俊,张娟.微生物法检测乳粉中叶酸的优化研究[J].中国乳业,2021(2):81-85.
[33] 李雪,王强,张磊.能力验证在实验室检测质量提升的应用研究[J].中国计量,2023,(6):78-81
[34] 刘健,陈雪,周明.能力验证对实验室内部质量管理的优化路径[J].质量技术监督研究,2023(3):29-32
[35] 周洁,冯晓丽,戴蕾蕾,等.实验室间比对在确保结果有效性中的应用[J].理化检验(化学分册),2022,58(1):103-105.
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