中国乳业 ›› 2025, Vol. 0 ›› Issue (1): 41-45.doi: 10.12377/1671-4393.25.01.08

• 疾病防治 • 上一篇    下一篇

一例犊牛呼吸道疾病的病原学诊断

袁文焕1, 陈静怡2, 张天琦2, 贾春涛2   

  1. 1 天津市武清区农业发展服务中心,天津 301700;
    2 国家奶牛产业技术体系武清综合试验站,天津 301718
  • 出版日期:2025-01-25 发布日期:2025-02-14
  • 作者简介:袁文焕（1972-）,女,天津人,本科,高级兽医师,研究方向为畜牧兽医技术推广;陈静怡（1997-）,女,天津人,硕士,研究方向为农业经济分析;张天琦（1997-）,男,天津人,硕士,研究方向为农业经济分析;贾春涛（1964-）,男,天津人,大专,研究方向为奶牛养殖及牧场管理。
  • 基金资助:
    国家奶牛产业技术体系项目（CARS-36-38）

A Case of Etiological Diagnosis of Bovine Respiratory Disease

YUAN Wenhuan1, CHEN Jingyi2, ZHANG Tianqi2, JIA Chuntao2   

  1. 1 Tianjin Wuqing District Agricultural Development Service Center, Tianjin 301700;
    2 Wuqing Comprehensive Experimental Station of National Dairy Cow Industry Technology System,Tianjin 301718
  • Online:2025-01-25 Published:2025-02-14

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摘要： [目的] 2023年8月,天津某牛场暴发以呼吸道症状为突出特征的传染病。为了确定病因,对发病犊牛进行相关病原的病原学诊断。[方法] 采集病死犊牛肺部病料,采用PCR方法对牛副流感病毒3型（BPIV-3）、牛呼吸道合胞体病毒（BRSV）、牛疱疹病毒1型（BHV-1）、牛腺病毒3型（BAdV-3）、牛分枝杆菌（Mycobacterium bovis）、牛肺炎克雷伯菌（Bovine Klebsiella pneumoniae）、牛支原体（Mycoplasma bovis）7 种牛呼吸道病原进行检测。[结果] 样本检测出BHV-1、BAdV-3共2 种病原,存在混合感染。其他病原未检出。[结论] 本研究证实BHV-1、BAdV-3在该牛场中存在和流行,为犊牛呼吸道疾病防控提供参考。

关键词: 牛疱疹病毒1型, 牛腺病毒3型, 病原学

Abstract: [Objective] In August 2023,an outbreak of infectious disease characterized by respiratory symptoms was reported on one cattle farm in Tianjin. To determine the cause of the disease,we conducted etiological diagnosis of the relevant pathogens in the affected calves. [Method] Lung specimens were collected from dead calves. We used the PCR method to detect seven bovine respiratory pathogens,including Bovine parainfluenza virus type 3,Bovine respiratory syncytial virus,Bovine herpesvirus type 1,Bovine adenovirus type 3,Mycobacterium bovis, Bovine Klebsiella bovis and Mycoplasma bovis. [Result] The results showed that two pathogens,BHV-1 and BAdV-3,were detected in the samples,and there was mixed infection. No other pathogens were detected. [Conclusion] This study confirmed the existence and prevalence of BHV-1 and BAdV-3 in dairy cows in this cattle farm,which provided reference for the prevention and control of respiratory diseases.

Key words: Bovine herpesvirus type 1, Bovine adenovirus type 3, etiology

[1] Baptista A L,Rezende A L,de Almeida Fonseca P,et al.Bovine respiratory disease complex associated mortality and morbidity rates in feedlot cattle from southeastern Brazil[J].The Journal of Infection in Developing Countries,2017,11(10):791-799.
[2] 刘志鹏,董秀梅,师东方,等.牛呼吸道疾病综合征的主要病原[J].畜牧兽医科技信息,2012(12):9-11.
[3] Narita M,Yamada M,Tsuboi T,et al.Immunohistopathology of calf pneumonia induced by endobronchial inoculation with bovine adenovirus 3[J].Veterinary Pathology,2002,39(5):565-571.
[4] Yamada M,Narita M,Nakamura K,et al.Apoptosis in calf pneumonia induced by endobronchial inoculation with bovine adenovirus type 3(BAV-3)[J].Journal of Comparative Pathology,2003,128(2/3):140-145.
[5] 何姝凡,岳华,汤承,等.牛腺病毒3型的研究进展[J].畜牧兽医学报,2022,53(4):1030-1040.
[6] Harrison S C.Principles of virus structure[J].Fields Virology,2001,1:53-85.
[7] Mayfield J E,Good P J,Vanoort H J,et al.Cloning and cleavage site mapping of DNA from bovine herpesvirus 1(cooper strain)[J].Journal of Virology,1983,47:259-264.
[8] Muylkens B,Thiry J,Kirten P,et al.Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis[J].Veterinary Research,2007,38(2):181-209.
[9] 郭长梅. 常见牛呼吸道疾病病因分析与综合防控探讨[J].中国乳业,2024(7):64-68.
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ISSN 1671-4393
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