为了解河南省长垣市奶山羊小反刍兽疫疫情流行情况和免疫抗体水平,2018—2020年,采用阻断酶联免疫吸附剂测定（ELISA）法和实时荧光聚合酶链式反应（PCR）法,对长垣市32 家奶山羊散养户采集羊血清和鼻拭子样品,进行羊小反刍兽疫免疫抗体及病原学检测。结果表明,2018年、2019年和2020年的血清抗体合格率分别为83.13%、85.64%和86.67%,平均85.17%,高于国家规定的70.00%。病原学监测全部样品为阴性。提示长垣市总体疫苗免疫效果良好,羊群具有抵抗小反刍兽疫侵袭的基本能力。为长垣市制定科学合理的防控措施、逐步消灭小反刍兽疫和退出小反刍兽疫免疫计划提供参考依据。

In order to understand the epidemic situation and immune antibody level of Peste des petits ruminants of dairy goats in Changyuan city,the blocking ELISA method and real-time PCR method were used to collect serum and nasal swab samples from 32 dairy goat free range households in Changyuan city from 2018 to 2020 to detect the immune antibody and etiology of Peste des petits ruminants.The results showed that: in 2018,2019 and 2020, the qualified rate of serum antibody was 83.13%,85.64% and 86.67 respectively,with an average of 85.17%,higher than 70.00% stipulated by the state.All samples were negative.It is suggested that the overall immune effect of vaccine in Changyuan city is good,and sheep have the basic ability to resist the invasion of Peste des petits ruminants.This study provides reference for formulating scientific and reasonable prevention and control measures,gradually eliminating peste des petits ruminants and withdrawing immunization plan of Peste des petits ruminants in Changyuan city.