为评估微生物法测定婴幼儿配方乳粉中叶酸含量的不确定度,结合《GB 5009.211—2014 食品安全国家标准 食品中叶酸的测定》及不确定度相关评估标准以及统计学方法,对婴幼儿配方乳粉中叶酸含量测定过程中的不确定度进行分析和评估。结果显示,婴幼儿配方乳粉中叶酸含量为（148.49±7.37） μg/100 g,k=2。其中,标准溶液和样液的配制过程是影响检测结果的主要因素,仪器测量偏差、重复性和检测方法是次要因素。表明微生物法测定婴幼儿配方乳粉中叶酸含量时不确定度较小,可用于婴幼儿配方乳粉中叶酸含量的测定。
To evaluate the uncertainty for determination of folic acid in infant formula by microbiological method. According to GB 5009.211—2014 National food safety standard-Determination of folic acid in food and relevant evaluation criteria of uncertainty and relevant statistical methods,the uncertainty components affecting folic acid content results in infant formula food were analysed and evaluated.The content of folic acid in infant formula was (148.49±7.37)μg/100g,k=2.It was found that the preparation process of standard solution and the preparation process of sample solution were the main factors affecting the test results.The deviation of instrument measurement, repeatability and test methods were secondary factors.The microbiological determination method of folic acid in infant formula has low uncertainty,It can be used for the determination of folic acid in infant formula.
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