为研究不同剂量25羟基维生素D3[25(OH)VD3]对断奶前犊牛血液水平、抗氧化能力和免疫功能的影响,选取18 头健康状况良好的新生荷斯坦犊牛,随机分成3 组,每组6 头进行为期56 天的饲养试验。分为对照组[不添加25(OH)VD3]、低剂量组[25(OH)VD3添加量6 000 IU/头·天]和高剂量组[25(OH)VD3添加量12 000 IU/头·天]。结果表明,与对照组相比,随着25(OH)VD3添加剂量增加,血浆生长激素、胰岛素样生长因子-1、过氧化氢酶、超氧化物歧化酶、总抗氧化能力以及免疫球蛋白G(IgG)含量显著增加(P<0.05),而丙二醛含量随剂量增加而存在降低趋势(P=0.079)。与对照组和低剂量组相比,高剂量组血浆免疫球蛋白A(IgA)含量有增加趋势(P=0.059)。综上所述,断奶前犊牛饲喂高剂量25(OH)VD3有利于促进体内生长激素和胰岛素样生长因子-1分泌,可提高犊牛抗氧化能力,增强免疫功能。
To study the effects of 25-hydroxyvitamin D3 [25(OH)VD3] at different doses on blood level, antioxidant capacity and immune function of pre-weaning calves,18 healthy Holstein calves were randomly assigned to 3 groups with 6 calves in each group for 56 days. They were divided into control group [0 IU 25(OH)VD3 per calf per day], low-dose group [6 000 IU 25(OH)VD3 per calf per day] and high-dose group [12 000 IU 25(OH)VD3 per calf per day]. The results showed that compared with the control group, the contents of plasma growth hormone, insulin-like growth factor-1, catalase, superoxide dismutase,total antioxidant capacity and immunoglobulin G (IgG) were significantly increased (P<0.05) with the increasing dose of 25(OH)VD3. The content of malondialdehyde decreased with the dosage of 25(OH)VD3 increased (P=0.079). Compared with the control group and the low-dose group, the plasma immunoglobulin A (IgA) content in the high-dose group had an increasing trend (P=0.059). In conclusion,feeding high doses of 25(OH)VD3 to calves before weaning can promote the secretion of growth hormone and insulin-like growth factor-1,improve antioxidant capacity, and enhance immune function.
[1] Nelson C D,Powell J L,Price D M,et al.Assessment of serum 25-hydroxyvitamin D concentrations of beef cows and calves across seasons and geographical locations[J]. Journal of Animal Science,2016,94(9):3958-3965.
[2] Celi P,Williams S,Engstrom M,et al.Safety evaluation of dietary levels of 25-hydroxyvitamin D 3 in growing calves[J]. Food and Chemical Toxicology,2018,111:641-649.
[3] Poindexter M B,Kweh M F,Zimpel R,et al.Feeding supplemental 25-hydroxyvitamin D3 increases serum mineral concentrations and alters mammary immunity of lactating dairy cows - ScienceDirect[J]. Journal of Dairy Science,2020,103(1): 805-822.
[4] Yang J,Tian G,Chen D,et al.Effects of dietary 25-hydroxyvitamin D3 supplementation on growth performance,immune function and antioxidative capacity in weaned piglets[J].Archives of Animal Nutrition,2019,73(1):44-51.
[5] Konowalchuk J D,Rieger A M,Kiemele M D,et al.Modulation of weanling pig cellular immunity in response to diet supplementation with 25-hydroxyvitamin D3[J]. Veterinary Immunology and Immunopathology,2013,155(1-2): 57-66.
[6] Sweeney B C,Rushen J,Weary D M,et al.Duration of weaning,starter intake,and weight gain of dairy calves fed large amounts of milk[J]. Journal of Dairy Science,2010,93(1): 148-152.
[7] Carroll J A,Arthington J D,Chase C C.Early weaning alters the acute-phase reaction to an endotoxin challenge in beef calves[J].Journal of Animal Science,2009,87(12): 4167-4172.
[8] Hypponen E,Boucher B,Berry D,et al.25-hydroxyvitamin D, IGF-1 and metabolic syndrome at age 45 years of age[J]. Diabetes,2008,57(2):298-305.
[9] 范炜,殷红,李春风,等.胰岛素样生长因子-1调节生长发育的研究进展[J]. 黑龙江畜牧兽医,2013,(1)﹕19-22,173.
[10] Wang EW,Siu PM,Pang MY,et al.Vitamin D deficiency,oxidative stress and antioxidant status:only weak association seen in the absence of advanced age,obesity or pre-existing disease[J]. British Journalof Nutrition,2017,118,(1): 11-16.
[11] Cavalcante I G D M,SilvaA S,Costa M J,et al.Effect of vitamin D3 supplementation and influence of BsmI polymorphism of the VDR gene of the inflammatory profile and oxidative stress in elderly women with vitamin D insufficiency:Vitamin D3 megadose reduces inflammatory markers[J]. Experimental Gerontology,2015,66:10-16.
[12] Maritim A C,Sanders R A,Watkins J B.Diabetes,oxidative stress and antioxidants:a review[J]. Journalof Biochemicaland Molecular Toxicology,2003,17(1): 24-38
[13] Tiwari B K,Pandey K B,Abidi A B,et al.Markers of oxidative stress during diabetes mellitus[J].Journal of Biomarkers,2013,1-8.
[14] Banakar M C,Paramasivan S K,Chattopadhyay M B,et al.1α,25-Dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital[J].World Journal of Gastroenterology,2004(9):1268-1275.
[15] Mokhtar S S,Yahaya S,Rasool A,et al.Calcitriol supplementation ameliorates microvascular endothelial dysfunction in Vitamin D-deficient diabetic rats by upregulating the vascular eNOS protein expression and reducing oxidative stress[J]. Oxidative Medicine and Cellular Longevity,2021,1-11.
[16] Nelson C D,Reinhardt T A,Thacker T C,et al.Modulation of the bovine innate immune response by production of 1alpha, 25-dihydroxyvitamin D(3)in bovine monocytes[J]. Journalof Dairy Science, 2010, 93(3):1041-1049.
[17] Konowalchuk J D,Rieger A M,Kiemele M D,et al.Modulation of weanling pig cellular immunity in response to diet supplementation with 25-hydroxyvitamin D3[J]. Veterinary Immunology and Immunopathology,2013,155(1-2): 57-66.
[18] Prosser D E,Jones G,Prosser D E,et al.Enzymes involved in the activation and inactivation of vitamin D[J]. Trends in Biochemical Sciences, 2005, 29(12): 664-673.
[19] Briere F,Servet-Delprat C,Bridon J M,et al.Human interleukin 10 induces naive surface immunoglobulin D+ (sIgD+) B cells to secrete IgG1 and IgG3[J]. Journal of Experimental Medical,1994,179(2):757-762.
[20] Martinez N,Rodney R M,Block E,et al.Effects of prepartum dietary cation-anion difference and source of vitamin D in dairy cows: lactation per-formance and energy metabolism[J]. Journal of Dairy Science,2017,101(3):2544-2562.
[21] Xu H,Zhang Q,Wang L,et al.Effects of 25-hydroxyvitamin d3 and oral calcium bolus on lactation performance,ca homeostasis,and health of multiparous dairy cows[J]. Animals,2021,11(6): 1576-1586.
