为了调查新疆地区某规模化奶牛场牛传染性鼻气管炎(IBR)发病情况,通过采集不同生长阶段牛群血清共计362 份,使用牛传染性鼻气管炎病毒gB(IBR-gB)抗体检测试剂盒检测牛传染性鼻气管炎病毒(IBRV)抗体效价,评估该奶牛场IBRV疫苗免疫效果。结果显示,后备牛中犊牛和青年牛IBRV抗体阳性率分别为88.57%(31/35)、75.00%(21/28);成年母牛中泌乳期母牛、干奶期母牛IBRV抗体阳性率分别为81.46%(145/178)、95.04%(115/121)。阴性数共44 份,可疑数8 份,IBRV抗体平均阳性率为88.38%;结果表明,疫苗接种后,不同生产阶段牛群均可产生不错的抗体保护效果,为奶牛场防控IBR提供依据。
In order to investigate the incidence of bovine infectious rhinotracheitis(IBR)on a large-scale dairy farm in Xinjiang,a total of 362 samples of bovine serum from different developmental stages were collected and the bovine infectious rhinotracheitis virus gB(IBR-gB)antibody detection reagent was used. The kit detects the antibody titer of the bovine infectious rhinotracheitis virus(IBRV),and evaluates the immunization effect of the IBRV vaccine on the dairy farm.The results showed that the positive rates of IBRV antibodies in the calves and young cattle were 88.57 %(31/35)and 75.00 %(21/28) respectively.Adult cows were lactating cows,and dry milk.The positive rates of IBRV antibody in cows were 81.46%(145/178),and 95.04%(115/121).There were 44 negatives and 8 suspicious ones.The average positive rate of IBRV antibody was 88.38%.The results showed that after vaccination,cattle at different stages of production can produce good antibody protection,providing a basis for dairy farms to prevent and control IBR.
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