中国乳业 ›› 2021, Vol. 0 ›› Issue (12): 90-93.doi: 10.12377/1671-4393.21.12.16

• 疾病防治 • 上一篇    下一篇

新疆某规模化奶牛场牛传染性鼻气管炎病毒抗体水平检测

王晨豫1, 魏勇2, 陈杰1, 曹梦园1, 陈明杰1, 齐亚银1,*   

  1. 1 石河子大学动物科技学院,新疆石河子 832000;
    2 新疆天润乳业股份有限公司,新疆乌鲁木齐 830000
  • 出版日期:2021-12-25 发布日期:2022-01-07
  • 通讯作者: *齐亚银(1980-),男,新疆石河子人,博士,教授,研究方向为动物传染病的诊断和防治。
  • 作者简介:王晨豫(1996-),男,宁夏石嘴山人,硕士,研究方向为动物临床诊断与防控技术;魏 勇(1980-),男,四川资中人,硕士,高级兽医师,研究方向为奶牛优质乳管理和良种繁育;陈 杰(1996-),男,新疆库尔勒人,硕士,研究方向为动物临床诊断与防控技术;曹梦园(1997-),男,新疆阿克苏人,硕士,研究方向为预防兽医学;陈明杰(1996-),男,新疆伊犁人,硕士,研究方向为预防兽医学。
  • 基金资助:
    新疆兵团十二师重大科技项目(SR2019002)

Detection and Prevention and Control of Antibody Level of Infectious Rhinotracheitis Virus in a Large-scale Dairy Farm in Xinjiang

WANG Chenyu1, WEI Yong2, CHEN Jie1, CAO Mengyuan1, CHEN Mingjie1, QI Yayin1,*   

  1. 1College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832000;
    2Xinjiang Tianrun Dairy Co.,LTD.,Urumqi Xinjiang 830000
  • Online:2021-12-25 Published:2022-01-07

摘要: 为了调查新疆地区某规模化奶牛场牛传染性鼻气管炎(IBR)发病情况,通过采集不同生长阶段牛群血清共计362 份,使用牛传染性鼻气管炎病毒gB(IBR-gB)抗体检测试剂盒检测牛传染性鼻气管炎病毒(IBRV)抗体效价,评估该奶牛场IBRV疫苗免疫效果。结果显示,后备牛中犊牛和青年牛IBRV抗体阳性率分别为88.57%(31/35)、75.00%(21/28);成年母牛中泌乳期母牛、干奶期母牛IBRV抗体阳性率分别为81.46%(145/178)、95.04%(115/121)。阴性数共44 份,可疑数8 份,IBRV抗体平均阳性率为88.38%;结果表明,疫苗接种后,不同生产阶段牛群均可产生不错的抗体保护效果,为奶牛场防控IBR提供依据。

关键词: 牛传染性鼻气管炎(IBR), 抗体水平, 酶联免疫吸附剂测定(ELISA)

Abstract: In order to investigate the incidence of bovine infectious rhinotracheitis(IBR)on a large-scale dairy farm in Xinjiang,a total of 362 samples of bovine serum from different developmental stages were collected and the bovine infectious rhinotracheitis virus gB(IBR-gB)antibody detection reagent was used. The kit detects the antibody titer of the bovine infectious rhinotracheitis virus(IBRV),and evaluates the immunization effect of the IBRV vaccine on the dairy farm.The results showed that the positive rates of IBRV antibodies in the calves and young cattle were 88.57 %(31/35)and 75.00 %(21/28) respectively.Adult cows were lactating cows,and dry milk.The positive rates of IBRV antibody in cows were 81.46%(145/178),and 95.04%(115/121).There were 44 negatives and 8 suspicious ones.The average positive rate of IBRV antibody was 88.38%.The results showed that after vaccination,cattle at different stages of production can produce good antibody protection,providing a basis for dairy farms to prevent and control IBR.

Key words: infectious bovine rhinotracheitis(IBR), antibody level, enzyme-linked Immunosorbent Assay(ELISA)

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