[目的]开发同时检测生乳中吡虫啉（Imidacloprid）、吡唑醚菌酯（Pyrazole ether ester）、氟虫腈（Fluorine worm nitrile）3 种农药残留的胶体金免疫层析的方法。[方法]采用柠檬酸三钠还原法制备胶体金溶液，冻干法制备胶体金标抗体冻干粉，置于微孔杯内，复溶后，吸取全部反应液于胶体金试纸条样品垫上，经层析，在免疫层析试纸条上显示结果。[结果]吡虫啉、吡唑醚菌酯、氟虫腈3 种农药检测检测下限分别为0.1 mg/kg，0.03mg/kg，0.02 mg/kg，保质期为1 年，符合《GB2763—2021食品安全国家标准 食品中农药最大残留限量》对生乳中农药残留做出明确限量规定吡虫啉、吡唑醚菌酯、氟虫腈最大残留量0.1 mg/kg，0.03 mg/kg，0.02 mg/kg要求。[结论]该方法操作简单，快速灵敏，稳定准确，可以作为前期农残检测的快速筛查手段。
[Objective] To develop a colloidal gold immunochromatography method for simultaneous detection of Imidacloprid，Pyrazole ether ester and Fluorine worm nitrile residues in raw milk. [Method] The colloidal gold solution was prepared by trisodium citrate reduction method, and the colloidal gold standard antibody lyophilized powder was prepared by lyophilization method. The powder was placed in a microporous cup，and the reaction solution was absorbed on the sample pad of colloidal gold test strip after resolution. [Result] The sensitivity of three pesticides，imidacloprid，pyrazoline and fipronil，were 0.1 mg/kg, 0.03mg/kg and 0.02 mg/kg，respectively. The shelf life was 1 year. In accordance with GB2763-2021 National Food Safety Standard Maximum Residue Limits for Pesticides in Food，the maximum residue limits of imidacloprid，pyrazolylosteril and fipronil in raw milk are specified to be 0.1mg/kg，0.03mg/kg and 0.02mg/kg respectively. [Conclusion] This method is simple, rapid，sensitive，stable and accurate，and can be used as a rapid screening method for early detection of agricultural residues.
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