[目的]调查新疆奎屯地区7 个规模化牧场奶牛副结核病的感染情况,提供科学有效防控措施。[方法]2019—2021年持续对奎屯地区7 个规模化牧场长期、顽固性腹泻牛只采集血液分离血清,使用ELISA检测法进行副结核病的检测。[结果]2019年总阳性率为6.55%（636/9 715）,2020年总阳性率为6.80%（705/10 375）,2021年总阳性率为5.23%（615/11 753）。[结论]检疫加淘汰的方法对降低牧场副结核病的阳性率有较好效果。
[Objective]Providing scientific and effective prevention and control measures for investigating the infection of paratuberculosis in dairy cows in 7 large-scale pastures in Kuitun area of Xinjiang.[Method]This experiment continued to collect blood from long-term and intractable diarrhea cows in 7 pastures in Kuitun area from 2019 to 2021,and used ELISA method to detect paratuberculosis.[Result] The overall positive rate in 2019 was 6.55%（636/9 715）,the overall positive rate in 2020 was 6.80% （705/10 375）,the overall positive rate in 2021 was 5.23%（615/11 753）.[Conclusion] The method of quarantine and elimination has good effect on reducing the positive rate of paratuberculosis in pasture.
[1] 孔繁德,徐淑菲,王景明,等. 副结核分枝杆菌诊断技术的研究进展[J]. 检验检疫学刊,2004,14(S1):57-60.
[2] 郑素琴,傅殿国,李翠云,等. 副结核菌苗接种牛和结核病牛对副结核菌素及结核菌素的变态反应[J].中国兽医科学,1988(5).
[3] 张丽媛,吴桐忠,马雪,等. 新疆北疆地区某规模化奶牛场奶牛副结核病的检测与分析[J].黑龙江畜牧兽医,2022(14):78-83.
[4] 努尔麦麦提·麦麦提敏. 新疆南疆牦牛副结核病的流行病学调查[D]. 阿拉尔:塔里木大学,2022.
[5] 陈志远,张宇名,迟丽丽,等. 山东省部分地区规模化奶牛场副结核病血清学调查[J].中国奶牛,2022(4):21-25.
[6] 赵怡雯,苍真伟,刘佳,等. 2018—2020年大连市规模奶牛场副结核抗体检测分析[J].饲料博览,2021(9):37-39.
[7] 师庆伟,桑学波,刁彩霞,等. 黑龙江省规模化奶牛场副结核病血清学调查[J].中国奶牛,2020(7):61-64.
[8] 王志刚,王庆泽,张宝玉. 奶牛副结核病的症状与预防措施[J].养殖技术顾问,2013(5):1.
